Details of Host Protein

 Host Protein General Information (ID: PT0199)
  Protein Name
ATP synthase F1 subunit beta (ATP5B)
  Gene Name
ATP5F1B
  Host Species
Homo sapiens
  Uniprot Entry Name
ATPB_HUMAN
  Protein Families
ATPase alpha/beta chains family
  EC Number
7.1.2.2
  Subcellular Location
Mitochondrion
  External Link
NCBI Gene ID
506
Uniprot ID
P06576
Ensembl ID
ENSG00000110955
HGNC ID
HGNC:830
  Function in Host
Mitochondrial membrane ATP synthase produces ATP from ADP in the presence of a proton gradientacross the membrane which is generated by electron transport complexesof the respiratory chain. F-type ATPases consist of two structuraldomains, F (1) - containing the extramembraneous catalytic core, andF (0) - containing the membrane proton channel, linked together by acentral stalk and a peripheral stalk. During catalysis, ATP synthesisin the catalytic domain of F (1) is coupled via a rotary mechanism ofthe central stalk subunits to proton translocation. Subunits alpha andbeta form the catalytic core in F (1). Rotation of the central stalkagainst the surrounding alpha (3) beta (3) subunits leads to hydrolysis ofATP in three separate catalytic sites on the beta subunits.
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  Related KEGG Pathway
Chemical carcinogenesis - reactive oxygen species hsa05208            Pathway Map 
Diabetic cardiomyopathy hsa05415            Pathway Map 
Oxidative phosphorylation hsa00190            Pathway Map 
Thermogenesis hsa04714            Pathway Map 
Metabolic pathways hsa01100            Pathway Map 
Alzheimer disease hsa05010            Pathway Map 
Parkinson disease hsa05012            Pathway Map 
Amyotrophic lateral sclerosis hsa05014            Pathway Map 
  3D Structure
AlphaFold ID: P06576 
  2D PNG Download  
  mmCIF File Download   
  PDB File Download   

Function of This Protein During Virus Infection
Virus NameSARS-COV-2 Protein Function Anti-viral [1]
Infected TissueLung Infection Time7-9 Days
Infected CellCalu-3 Cells (Human epithelial cell line) Cellosaurus IDCVCL_0609 
Method DescriptionTo detect the role of host protein ATP5F1B in viral infection, ATP5F1B protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen.
ResultsIt is reported that knockout of ATP5F1B increases SARS-CoV-2 RNA levels compared with control group.

 Full List of Virus RNA Interacting with This Protien
            RNA Region: 3'-UTR (hCoV-19/IPBCAMS-YL01/2020 )
              RNA Region Details RNA Info Click to show the detail information of this RNA binding region [2]
              Strains Name
hCoV-19/IPBCAMS-YL01/2020
              Strains Family
Beta (B.1.351)
              RNA Binding Region
3'-UTR
              Virus Name
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
              Interaction Type Unlikely to be direct binder
              Infection Cells Huh7.5.1 cells (Hepatocyte derived cellular carcinoma cell)  (CVCL_E049 )
              Cell Originated Tissue Liver
              Infection Time 30 h
              Interaction Score MIST = 0.623095691
              Method Description comprehensive identification of RNA-binding proteins by massspectrometry (ChIRP-MS)

Differential Gene Expression During SARS-COV-2 Infection
GEO Accession: GSE152641
Sample Type: Blood
Samples Details: Healthy Control: 24; COVID-19: 62
Platform: GPL24676 Illumina NovaSeq 6000
  PNG Download  
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GEO Accession: GSE162835
Sample Type: Nasopharyngeal Swabs
Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3
Platform: GPL24676 Illumina NovaSeq 6000
  PNG Download  
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GEO Accession: GSE175779
Sample Type: Human Bronchial Epithelial Cells
Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)
Platform: GPL18573 Illumina NextSeq 500
  PNG Download  
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Potential Drug(s) that Targets This Protein
Drug Name DrunkBank ID Pubchem ID TTD ID REF
AKOS013569828 DB07384  5287692  . [3]
AUROVERTINB DB07394  444853  . [3]
N1-(2-AMINO-4-METHYLPENTYL)OCTAHYDRO-PYRROLO[1,2-A]PYRIMIDINE DB08629  5289474  . [3]
Phenylaethylsenfoel DB12695  16741  D07AOL  [3]
Piceatannol DB08399  667639  D0O0VZ  [3]
Quercetin DB04216  5280343  D0K8KX  [3]

Protein Sequence Information
MLGFVGRVAAAPASGALRRLTPSASLPPAQLLLRAAPTAVHPVRDYAAQTSPSPKAGAATGRIVAVIGAVVDVQFDEGLPPILNALEVQGRETRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVLDSGAPIKIPVGPETLGRIMNVIGEPIDERGPIKTKQFAPIHAEAPEFMEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS
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References
1 Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2. Nat Commun. 2022 Apr 25;13(1):2237.
2 Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. Cell Res. 2022 Jan;32(1):9-23.
3 Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis. EMBO J. 2021 Sep 1;40(17):e107776.