Details of Host Protein
Host Protein General Information (ID: PT0274) | |||||||||
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Protein Name |
Cleavage factor Im complex 25 kDa subunit (CFIm25)
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Gene Name |
NUDT21
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Host Species |
Homo sapiens
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Uniprot Entry Name |
CPSF5_HUMAN
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Protein Families |
Nudix hydrolase family
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Subcellular Location |
Nucleus Cytoplasm
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External Link | |||||||||
NCBI Gene ID | |||||||||
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Ensembl ID | |||||||||
HGNC ID | |||||||||
Function in Host |
Component of the cleavage factor Im (CFIm) complex thatfunctions as an activator of the pre-mRNA 3'-end cleavage andpolyadenylation processing required for the maturation of pre-mRNA intofunctional mRNAs. CFIm contributes to the recruitment of multiprotein complexes onspecific sequences on the pre-mRNA 3'-end, so called cleavage andpolyadenylation signals (pA signals). Most pre-mRNAs contain multiple pAsignals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a keyregulator of cleavage and polyadenylation site choice during APAthrough its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. NUDT21/CPSF5activates indirectly the mRNA 3'-processing machinery by recruitingCPSF6 and/or CPSF7. Binds to 5'-UGUA-3' elementslocalized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing. The homodimermediates simultaneous sequence-specific recognition of two 5'-UGUA-3'elements within the pre-mRNA. Playsa role in somatic cell fate transitions and pluripotency by regulatingwidespread changes in gene expression through an APA-dependent function. Binds to chromatin. Binds to, but doesnot hydrolyze mono- and di-adenosine nucleotides.
[1-8]
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Related KEGG Pathway | |||||||||
mRNA surveillance pathway | hsa03015 | Pathway Map | |||||||
3D Structure |
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Function of This Protein During Virus Infection | |||||||||
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Virus Name | SARS-COV-2 | Protein Function | Anti-viral | [9] | |||||
Infected Tissue | Lung | Infection Time | 7-9 Days | ||||||
Infected Cell | Calu-3 Cells (Human epithelial cell line) | Cellosaurus ID | CVCL_0609 | ||||||
Method Description | To detect the role of host protein NUDT21 in viral infection, NUDT21 protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen. | ||||||||
Results | It is reported that knockout of NUDT21 increases SARS-CoV-2 RNA levels compared with control group. |
Full List of Virus RNA Interacting with This Protien | |||||||||
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RNA Region: Not Specified Virus Region (hCoV-19/France/IDF-220-95/2020 ) | |||||||||
RNA Region Details | RNA Info Click to show the detail information of this RNA binding region | [10] | |||||||
Strains Name |
hCoV-19/France/IDF-220-95/2020
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RNA Binding Region |
Not Specified Virus Region
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Virus Name |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
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Interaction Type | Direct interaction | ||||||||
Infection Cells | HEK293 Cells (Human embryonic kidney cell) (CVCL_0045 ) | ||||||||
Cell Originated Tissue | Kidney | ||||||||
Infection Time | 48 h | ||||||||
Interaction Score | SAINT score ≥ 0.79 | ||||||||
Method Description | comprehensive identification of RNA-binding proteins by massspectrometry (ChIRP-MS) |