Details of Host Protein

Host Protein General Information (ID: PT0380)
Protein Name	Electron transfer flavoprotein subunit beta (ETFB)	Gene Name	ETFB
Host Species	Homo sapiens	Uniprot Entry Name	ETFB_HUMAN
Protein Families	ETF beta-subunit/FixA family
Subcellular Location	Mitochondrion matrix
External Link
	NCBI Gene ID	2109
	Uniprot ID	P38117
	Ensembl ID	ENSG00000105379
	HGNC ID	HGNC:3482
Function in Host	Heterodimeric electron transfer flavoprotein that acceptselectrons from several mitochondrial dehydrogenases, including acyl-CoAdehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers theelectrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase. Required for normal mitochondrialfatty acid oxidation and normal amino acid metabolism. ETFB binds an AMP molecule that probably has a purelystructural role. [1-7] Click to Show/Hide
3D Structure	PDB ID: 1EFV FASTA Download 2D PNG Download PDB File Download

Function of This Protein During Virus Infection
Virus Name	SARS-COV-2	Protein Function	Pro-viral	[8]
Infected Tissue	Colon	Infection Time	48 h
Infected Cell	Caco-2 cells (Human colorectal adenocarcinoma cell)	Cellosaurus ID	CVCL_0025
Method Description	To detect the role of host protein ETFB in viral infection, ETFB protein knockout Caco-2 cells were infected with SARS-COV-2 for 48 h , and the effects on infection was detected through qPCR.
Results	It is reported that Knockdown of ETFB leads to the reduced the vRNA levels compared with control group.

Full List of Virus RNA Interacting with This Protien
*RNA Region: 3'-UTR (hCoV-19/IPBCAMS-YL01/2020 )*
RNA Region Details	RNA Info Click to show the detail information of this RNA binding region	[8]
Strains Name	hCoV-19/IPBCAMS-YL01/2020
Strains Family	Beta (B.1.351)
RNA Binding Region	3'-UTR
Virus Name	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Interaction Type	Unlikely to be direct binder
Infection Cells	Huh7.5.1 cells (Hepatocyte derived cellular carcinoma cell) (CVCL_E049 )
Cell Originated Tissue	Liver
Infection Time	30 h
Interaction Score	MIST = 0.719569937
Method Description	comprehensive identification of RNA-binding proteins by massspectrometry (ChIRP-MS)

Differential Gene Expression During SARS-COV-2 Infection

GEO Accession: GSE152641

Sample Type: Blood

Samples Details: Healthy Control: 24; COVID-19: 62

Platform: GPL24676 Illumina NovaSeq 6000

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GEO Accession: GSE162835

Sample Type: Nasopharyngeal Swabs

Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3

Platform: GPL24676 Illumina NovaSeq 6000

PNG Download

GEO Accession: GSE175779

Sample Type: Human Bronchial Epithelial Cells

Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)

Platform: GPL18573 Illumina NextSeq 500

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Protein Phosphorylation after Virus Infection
T194 [9] PNG Download

Potential Drug(s) that Targets This Protein
Drug Name																									DrunkBank ID																				Pubchem ID																				TTD ID																									REF
SIROLIMUS																									DB00877																				5284616																				D03LJR																									[8]

Protein Sequence Information
MAELRVLVAVKRVIDYAVKIRVKPDRTGVVTDGVKHSMNPFCEIAVEEAVRLKEKKLVKEVIAVSCGPAQCQETIRTALAMGADRGIHVEVPPAEAERLGPLQVARVLAKLAEKEKVDLVLLGKQAIDDDCNQTGQMTAGFLDWPQGTFASQVTLEGDKLKVEREIDGGLETLRLKLPAVVTADLRLNEPRYATLPNIMKAKKKKIEVIKPGDLGVDLTSKLSVISVEDPPQRTAGVKVETTEDLVAKLKEIGRI Click to Show/Hide

References
1	Human METTL20 is a mitochondrial lysine methyltransferase that targets the B subunit of electron transfer flavoprotein (ETFB) and modulates its activity. J Biol Chem. 2015 Jan 2;290(1):423-34.
2	Three-dimensional structure of human electron transfer flavoprotein to 2.1-A resolution. Proc Natl Acad Sci USA. 1996 Dec 10;93(25):14355-60.
3	Mutations and polymorphisms of the gene encoding the beta-subunit of the electron transfer flavoprotein in three patients with glutaric acidemia type II. Hum Mol Genet. 1994 Mar;3(3):429-35.
4	Dynamics driving function: new insights from electron transferring flavoproteins and partner complexes. FEBS J. 2007 Nov;274(21):5481-504.
5	Stabilization of non-productive conformations underpins rapid electron transfer to electron-transferring flavoprotein. J Biol Chem. 2005 Aug 26;280(34):30361-6.
6	Extensive domain motion and electron transfer in the human electron transferring flavoprotein.medium chain Acyl-CoA dehydrogenase complex. J Biol Chem. 2004 Jul 30;279(31):32904-12.
7	Clear relationship between ETF/ETFDH genotype and phenotype in patients with multiple acyl-CoA dehydrogenation deficiency. Hum Mutat. 2003 Jul;22(1):12-23.
8	Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. Cell Res. 2022 Jan;32(1):9-23.
9	Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.