Details of Host Protein
Host Protein General Information (ID: PT0576) | |||||||||
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Protein Name |
PKR-associating protein X (PACT)
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Gene Name |
PRKRA
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Host Species |
Homo sapiens
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Uniprot Entry Name |
PRKRA_HUMAN
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Protein Families |
PRKRA family
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Subcellular Location |
Cytoplasm; perinuclear region
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External Link | |||||||||
NCBI Gene ID | |||||||||
Uniprot ID | |||||||||
Ensembl ID | |||||||||
HGNC ID | |||||||||
Function in Host |
Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibitionof translation and induction of apoptosis. Required for siRNAproduction by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required forprocessing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in aEIF2AK2/PKR-dependent manner.
[1-6]
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3D Structure |
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Full List of Virus RNA Interacting with This Protien | |||||||||
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RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain ) | |||||||||
RNA Region Details |
RNA Info
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[7] | |||||||
Strains Name |
hCoV-19/Not Specified Virus Strain
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Strains Family |
Beta (B.1.351)
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RNA Binding Region |
ORF10
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Virus Name |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
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Infection Cells | Huh7 cells (human liver cell line); Calu-3 cells (human lung cancer cell line) (CVCL_0336;CVCL_0609 ) | ||||||||
Cell Originated Tissue | Liver; Lung | ||||||||
Interaction Score | P-value < 0.05 | ||||||||
Method Description | RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS) |
Differential Gene Expression During SARS-COV-2 Infection | |||||||||||||||||||||||||||||||||||||||||
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Protein Phosphorylation after Virus Infection | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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S18
[8] |
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S22
[8] |
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T20
[8] |
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Protein Sequence Information | ||||||||||||||
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MSQSRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCTWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK
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