Details of Host Protein

 Host Protein General Information (ID: PT0651)
  Protein Name
Mitotic checkpoint protein BUB3 (BUB3)
  Gene Name
BUB3
  Host Species
Homo sapiens
  Uniprot Entry Name
BUB3_HUMAN
  Protein Families
WD repeat BUB3 family
  Subcellular Location
Nucleus
  External Link
NCBI Gene ID
9184
Uniprot ID
O43684
Ensembl ID
ENSG00000154473
HGNC ID
HGNC:1151
  Function in Host
Has a dual function in spindle-assembly checkpoint signalingand in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolarattachments. Necessary for kinetochore localization of BUB1. Regulateschromosome segregation during oocyte meiosis. The BUB1/BUB3 complexplays a role in the inhibition of anaphase-promoting complex orcyclosome (APC/C) when spindle-assembly checkpoint is activated andinhibits the ubiquitin ligase activity of APC/C by phosphorylating itsactivator CDC20. This complex can also phosphorylate MAD1L1.
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  Related KEGG Pathway
Human T-cell leukemia virus 1 infection hsa05166            Pathway Map 
Cell cycle hsa04110            Pathway Map 
  3D Structure
AlphaFold ID: O43684 
  2D PNG Download  
  mmCIF File Download   
  PDB File Download   

 Full List of Virus RNA Interacting with This Protien
            RNA Region: Not Specified Virus Region (hCoV-19/France/IDF-220-95/2020 )
              RNA Region Details RNA Info Click to show the detail information of this RNA binding region [1]
              Strains Name
hCoV-19/France/IDF-220-95/2020
              RNA Binding Region
Not Specified Virus Region
              Virus Name
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
              Interaction Type Direct interaction
              Infection Cells HEK293 Cells (Human embryonic kidney cell)  (CVCL_0045 )
              Cell Originated Tissue Kidney
              Infection Time 48 h
              Interaction Score SAINT score ≥ 0.79
              Method Description comprehensive identification of RNA-binding proteins by massspectrometry (ChIRP-MS)

Differential Gene Expression During SARS-COV-2 Infection
GEO Accession: GSE152641
Sample Type: Blood
Samples Details: Healthy Control: 24; COVID-19: 62
Platform: GPL24676 Illumina NovaSeq 6000
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GEO Accession: GSE162835
Sample Type: Nasopharyngeal Swabs
Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3
Platform: GPL24676 Illumina NovaSeq 6000
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GEO Accession: GSE175779
Sample Type: Human Bronchial Epithelial Cells
Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)
Platform: GPL18573 Illumina NextSeq 500
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Protein Sequence Information
MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPEVNVMVTGSWDQTVKLWDPRTPCNAGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESSLKYQTRCIRAFPNKQGYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKSPCT
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References
1 Characterization and functional interrogation of the SARS-CoV-2 RNA interactome. Cell Rep. 2022 Apr 26;39(4):110744.