Details of Host Protein

KEGG: hsa05165 Pathway Download ×

KEGG: hsa05203 Pathway Download ×

KEGG: hsa05230 Pathway Download ×

KEGG: hsa00010 Pathway Download ×

KEGG: hsa00620 Pathway Download ×

KEGG: hsa04930 Pathway Download ×

KEGG: hsa04922 Pathway Download ×

KEGG: hsa01100 Pathway Download ×

Host Protein General Information (ID: PT0888)
Protein Name	Pyruvate kinase M2 (PKM)	Gene Name	PKM
Host Species	Homo sapiens	Uniprot Entry Name	KPYM_HUMAN
Protein Families	Pyruvate kinase family
EC Number	2.7.1.4.
Subcellular Location	Cytoplasm Nucleus
External Link
	NCBI Gene ID	5315
	Uniprot ID	P14618
	Ensembl ID	ENSG00000067225
	HGNC ID	HGNC:9021
Function in Host	Catalyzes the final rate-limiting step of glycolysis bymediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric formand nearly inactive dimeric form determines whether glucose carbons arechanneled to biosynthetic processes or used for glycolytic ATPproduction. Thetransition between the 2 forms contributes to the control of glycolysisand is important for tumor cell proliferation and survival. [1-4] Click to Show/Hide
Related KEGG Pathway
	Human papillomavirus infection	hsa05165		Pathway Map
	Viral carcinogenesis	hsa05203		Pathway Map
	Central carbon metabolism in cancer	hsa05230		Pathway Map
	Glycolysis / Gluconeogenesis	hsa00010		Pathway Map
	Pyruvate metabolism	hsa00620		Pathway Map
	Type II diabetes mellitus	hsa04930		Pathway Map
	Glucagon signaling pathway	hsa04922		Pathway Map
	Metabolic pathways	hsa01100		Pathway Map
3D Structure	PDB ID: 1T5A FASTA Download 2D PNG Download PDB File Download

Full List of Virus RNA Interacting with This Protien
*RNA Region: 3'-UTR (hCoV-19/IPBCAMS-YL01/2020 )*
RNA Region Details	RNA Info Click to show the detail information of this RNA binding region	[5]
Strains Name	hCoV-19/IPBCAMS-YL01/2020
Strains Family	Beta (B.1.351)
RNA Binding Region	3'-UTR
Virus Name	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Interaction Type	Unlikely to be direct binder
Infection Cells	Huh7.5.1 cells (Hepatocyte derived cellular carcinoma cell) (CVCL_E049 )
Cell Originated Tissue	Liver
Infection Time	30 h
Interaction Score	MIST = 0.68551316
Method Description	comprehensive identification of RNA-binding proteins by massspectrometry (ChIRP-MS)

Differential Gene Expression During SARS-COV-2 Infection

GEO Accession: GSE152641

Sample Type: Blood

Samples Details: Healthy Control: 24; COVID-19: 62

Platform: GPL24676 Illumina NovaSeq 6000

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GEO Accession: GSE162835

Sample Type: Nasopharyngeal Swabs

Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3

Platform: GPL24676 Illumina NovaSeq 6000

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GEO Accession: GSE175779

Sample Type: Human Bronchial Epithelial Cells

Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)

Platform: GPL18573 Illumina NextSeq 500

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Protein Phosphorylation after Virus Infection
S100 [6] PNG Download
S249 [6] PNG Download
S37 [7] PNG Download
S77 [7] PNG Download
S77 [6] PNG Download
S97 [6] PNG Download
T80 [6] PNG Download

Protein Sequence Information
MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP Click to Show/Hide

References
1	Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1. Cell. 2011 May 27;145(5):732-44.
2	Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcription. Int J Biochem Cell Biol. 2008;40(5):1043-54.
3	Structural basis for tumor pyruvate kinase M2 allosteric regulation and catalysis. Biochemistry. 2005 Jul 12;44(27):9417-29.
4	An in vitro novel mechanism of regulating the activity of pyruvate kinase M2 by thyroid hormone and fructose 1, 6-bisphosphate. Biochemistry. 1991 Jul 23;30(29):7105-11.
5	Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. Cell Res. 2022 Jan;32(1):9-23.
6	Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.
7	The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell. 2020 Aug 6;182(3):685-712.e19.