Functions to regulate alternative splicing in neurons bybinding pre-mRNA in a sequence-specific manner to activate exoninclusion or exclusion. It binds specifically to thesequences 5'-YCAY-3' and regulates splicing in only a subset ofregulated exons. Binding to an exonic 5'-YCAY-3'cluster changes the protein complexes assembled on pre-mRNA, blockingU1 snRNP binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhances spliceosome assembly and exon inclusion. WithNOVA1, they perform unique biological functions in different brainareas and cell types. Uniquely regulates alternative splicing events ofa series of axon guidance related genes during cortical development, being essential for central nervous system development by regulatingneural networks wiring. Regulates differentially alternative splicingon the same transcripts expressed in different neurons. This includesfunctional differences in transcripts expressed in cortical andcerebellar excitatory versus inhibitory neurons where is required for, respectively, development of laminar structure and motor coordinationand synapse formation. Also the regulation the regulation of intronretention can sequester the trans-acting splicing factor PTBP2, actingas a variable cis-acting scaffolding platform for PTBP2 across variousnatural conditions. [1-2]

MEPEAPDSRKRPLETPPEVVCTKRSNTGEEGEYFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGTTERVCLVQGTAEALNAVHSFIAEKVREIPQAMTKPEVVNILQPQTTMNPDRAKQAKLIVPNSTAGLIIGKGGATVKAVMEQSGAWVQLSQKPEGINLQERVVTVSGEPEQVHKAVSAIVQKVQEDPQSSSCLNISYANVAGPVANSNPTGSPYASPADVLPAAAAASAAAASGLLGPAGLAGVGAFPAALPAFSGTDLLAISTALNTLASYGYNTNSLGLGLNSAAASGVLAAVAAGANPAAAAAANLLASYAGEAGAGPAGGAAPPPPPPPGALGSFALAAAANGYLGAGAGGGAGGGGGPLVAAAAAAGAAGGFLTAEKLAAESAKELVEIAVPENLVGAILGKGGKTLVEYQELTGARIQISKKGEFLPGTRNRRVTITGSPAATQAAQYLISQRVTYEQGVRASNPQKVG