Details of Host Protein

KEGG: hsa05171 Pathway Download ×

KEGG: hsa03010 Pathway Download ×

Host Protein General Information (ID: PT0111)
Protein Name	60S ribosomal protein L28 (RPL27)	Gene Name	RPL27
Host Species	Homo sapiens	Uniprot Entry Name	RL27_HUMAN
Protein Families	Eukaryotic ribosomal protein eL27 family
Subcellular Location	Cytoplasm; cytosol Cytoplasm Rough endoplasmic reticulum
External Link
	NCBI Gene ID	6155
	Uniprot ID	P61353
	Ensembl ID	ENSG00000131469
	HGNC ID	HGNC:10328
Function in Host	Component of the large ribosomal subunit. Required for properrRNA processing and maturation of 28S and 5. 8S rRNAs. [1-5] Click to Show/Hide
Related KEGG Pathway
	Coronavirus disease - COVID-19	hsa05171		Pathway Map
	Ribosome	hsa03010		Pathway Map
3D Structure	PDB ID: 4UG0 FASTA Download 2D PNG Download PDB File Download

Host Protein - Virus RNA Network

Network Download

Full List of Virus RNA Interacting with This Protien
*RNA Region: 3'-UTR (hCoV-19/IPBCAMS-YL01/2020 )*
RNA Region Details	RNA Info Click to show the detail information of this RNA binding region	[6]
Strains Name	hCoV-19/IPBCAMS-YL01/2020
Strains Family	Beta (B.1.351)
RNA Binding Region	3'-UTR
Virus Name	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Interaction Type	Potiential to be direct binder
Interaction Binding Type	Single stranded RNA binding
Infection Cells	Huh7.5.1 cells (Hepatocyte derived cellular carcinoma cell) (CVCL_E049 )
Cell Originated Tissue	Liver
Infection Time	30 h
Interaction Score	MIST = 0.685522534
Method Description	comprehensive identification of RNA-binding proteins by massspectrometry (ChIRP-MS)
*RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain )*
RNA Region Details	RNA Info Click to show the detail information of this RNA binding region	[7]
Strains Name	hCoV-19/Not Specified Virus Strain
RNA Binding Region	ORF10
Virus Name	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Infection Cells	Huh7 cells (human liver cell line) Huh7 cells (human liver cell line) (CVCL_0336 )
Cell Originated Tissue	Liver
Interaction Score	P-value < 0.05
Method Description	RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS)

Differential Gene Expression During SARS-COV-2 Infection

GEO Accession: GSE152641

Sample Type: Blood

Samples Details: Healthy Control: 24; COVID-19: 62

Platform: GPL24676 Illumina NovaSeq 6000

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GEO Accession: GSE162835

Sample Type: Nasopharyngeal Swabs

Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3

Platform: GPL24676 Illumina NovaSeq 6000

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GEO Accession: GSE175779

Sample Type: Human Bronchial Epithelial Cells

Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)

Platform: GPL18573 Illumina NextSeq 500

PNG Download

Protein Sequence Information
MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF Click to Show/Hide

References
1	Structural snapshots of actively translating human ribosomes. Cell. 2015 May 7;161(4):845-57.
2	Loss of function mutations in RPL27 and RPS27 identified by whole-exome sequencing in Diamond-Blackfan anaemia. Br J Haematol. 2015 Mar;168(6):854-64.
3	Structure of the human 80S ribosome. Nature. 2015 Apr 30;520(7549):640-5.
4	Structures of the human and Drosophila 80S ribosome. Nature. 2013 May 2;497(7447):80-5.
5	Characterization and analysis of posttranslational modifications of the human large cytoplasmic ribosomal subunit proteins by mass spectrometry and Edman sequencing. J Protein Chem. 2003 Apr;22(3):249-58.
6	Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. Cell Res. 2022 Jan;32(1):9-23.
7	Mapping the host protein interactome of non-coding regions in SARS-CoV-2 genome. bioRxiv. 2021 Jun; DOI:10.1101/2021.06.19.449092.