Details of Host Protein

 Host Protein General Information (ID: PT0141)
  Protein Name
Actin, cytoplasmic 1 (ACTB)
  Gene Name
ACTB
  Host Species
Homo sapiens
  Uniprot Entry Name
ACTB_HUMAN
  Protein Families
Actin family
  Subcellular Location
Cytoplasm; cytoskeleton Nucleus
  External Link
NCBI Gene ID
60
Uniprot ID
P60709
Ensembl ID
ENSG00000075624
HGNC ID
HGNC:132
  Function in Host
Actin is a highly conserved protein that polymerizes toproduce filaments that form cross-linked networks in the cytoplasm ofcells. Actin exists in both monomeric (G-actin) andpolymeric (F-actin) forms, both forms playing key functions, such ascell motility and contraction. In addition to theirrole in the cytoplasmic cytoskeleton, G- and F-actin also localize inthe nucleus, and regulate gene transcription and motility and repair ofdamaged DNA. [1-2]
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  Related KEGG Pathway
Platelet activation hsa04611            Pathway Map 
Neutrophil extracellular trap formation hsa04613            Pathway Map 
Leukocyte transendothelial migration hsa04670            Pathway Map 
Influenza A hsa05164            Pathway Map 
Viral myocarditis hsa05416            Pathway Map 
Bacterial invasion of epithelial cells hsa05100            Pathway Map 
Vibrio cholerae infection hsa05110            Pathway Map 
Pathogenic Escherichia coli infection hsa05130            Pathway Map 
  3D Structure
PDB ID: 3BYH 
FASTA   Download
  2D PNG Download  
PDB File Download

Host Protein - Virus RNA Network
  Network Download  

 Full List of Virus RNA Interacting with This Protien
            RNA Region: 3'-UTR (hCoV-19/IPBCAMS-YL01/2020 )
              RNA Region Details RNA Info Click to show the detail information of this RNA binding region [3]
              Strains Name
hCoV-19/IPBCAMS-YL01/2020
              Strains Family
Beta (B.1.351)
              RNA Binding Region
3'-UTR
              Virus Name
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
              Interaction Type Unlikely to be direct binder
              Infection Cells Huh7.5.1 cells (Hepatocyte derived cellular carcinoma cell)  (CVCL_E049 )
              Cell Originated Tissue Liver
              Infection Time 30 h
              Interaction Score MIST = 0.691965934
              Method Description comprehensive identification of RNA-binding proteins by massspectrometry (ChIRP-MS)
           RNA Region: Not Specified Virus Region (hCoV-19/Not Specified Virus Strain )
              RNA Region Details RNA Info Click to show the detail information of this RNA binding region [4]
              Strains Name
hCoV-19/Not Specified Virus Strain
              RNA Binding Region
Not Specified Virus Region
              Virus Name
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
              Infection Cells Huh7 cells (liver carcinoma cell) Huh7 cells (liver carcinoma cell)  (CVCL_0336 )
              Cell Originated Tissue Liver
              Infection Time 24h
              Interaction Score log2FC = 4.71103E+14
              Method Description RNA antisense purification and quantitative mass spectrometry (RAP-MS); Tandem mass tag (TMT) labelling; liquid chromatography tandem mass spectrometry (LC-MS/MS); Westernblot

Differential Gene Expression During SARS-COV-2 Infection
GEO Accession: GSE152641
Sample Type: Blood
Samples Details: Healthy Control: 24; COVID-19: 62
Platform: GPL24676 Illumina NovaSeq 6000
  PNG Download  
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GEO Accession: GSE162835
Sample Type: Nasopharyngeal Swabs
Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3
Platform: GPL24676 Illumina NovaSeq 6000
  PNG Download  
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GEO Accession: GSE175779
Sample Type: Human Bronchial Epithelial Cells
Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)
Platform: GPL18573 Illumina NextSeq 500
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Protein Phosphorylation after Virus Infection
S199 [5]
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S234 [5]
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S235 [5]
  PNG Download  
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S323 [6]
  PNG Download  
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S33 [6]
  PNG Download  
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S368 [5]
  PNG Download  
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S52 [6]
  PNG Download  
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Y218 [5]
  PNG Download  
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Protein Sequence Information
MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
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References
1 NAA80 is actin s N-terminal acetyltransferase and regulates cytoskeleton assembly and cell motility. Proc Natl Acad Sci USA. 2018 Apr 24;115(17):4399-4404.
2 Nuclear ARP2/3 drives DNA break clustering for homology-directed repair. Nature. 2018 Jul;559(7712):61-66.
3 Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. Cell Res. 2022 Jan;32(1):9-23.
4 The SARS-CoV-2 RNA-protein interactome in infected human cells. Nat Microbiol. 2021 Mar;6(3):339-353.
5 Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.
6 The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell. 2020 Aug 6;182(3):685-712.e19.