Details of Host Protein

KEGG: hsa00350 Pathway Download ×

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KEGG: hsa04936 Pathway Download ×

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Host Protein General Information (ID: PT0166)
Protein Name	Alcohol dehydrogenase 5 (FALDH)	Gene Name	ADH5
Host Species	Homo sapiens	Uniprot Entry Name	ADHX_HUMAN
Protein Families	Zinc-containing alcohol dehydrogenase family
EC Number	1.1.1.1; 1.1.1.-; 1.1.1.284
Subcellular Location	Cytoplasm
External Link
	NCBI Gene ID	128
	Uniprot ID	P11766
	Ensembl ID	ENSG00000197894
	HGNC ID	HGNC:253
Function in Host	Catalyzes the oxidation of long-chain primary alcohols andthe oxidation of S- (hydroxymethyl) glutathione. Alsooxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and theend product, a dicarboxylic acid, (5Z, 8Z, 11Z, 14Z) -eicosatetraenedioate. Class-III ADH is remarkably ineffective in oxidizingethanol. [1-2] Click to Show/Hide
Related KEGG Pathway
	Tyrosine metabolism	hsa00350		Pathway Map
	Glycolysis / Gluconeogenesis	hsa00010		Pathway Map
	Pyruvate metabolism	hsa00620		Pathway Map
	Alcoholic liver disease	hsa04936		Pathway Map
	Metabolic pathways	hsa01100		Pathway Map
	Carbon metabolism	hsa01200		Pathway Map
	Fatty acid degradation	hsa00071		Pathway Map
	Retinol metabolism	hsa00830		Pathway Map
3D Structure	PDB ID: 1M6H FASTA Download 2D PNG Download PDB File Download

Function of This Protein During Virus Infection
Virus Name	SARS-COV-2	Protein Function	Anti-viral	[3]
Infected Tissue	Lung	Infection Time	7-9 Days
Infected Cell	Calu-3 Cells (Human epithelial cell line)	Cellosaurus ID	CVCL_0609
Method Description	To detect the role of host protein ADH5 in viral infection, ADH5 protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen.
Results	It is reported that knockout of ADH5 increases SARS-CoV-2 RNA levels compared with control group.

Full List of Virus RNA Interacting with This Protien
*RNA Region: Not Specified Virus Region (hCoV-19/USA/WA1/2020 )*
RNA Region Details	RNA Info Click to show the detail information of this RNA binding region	[4]
Strains Name	hCoV-19/USA/WA1/2020
Strains Family	Alpha (B.1.1.7)
RNA Binding Region	Not Specified Virus Region
Virus Name	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Infection Cells	Huh7.5 cells (Hepatocyte derived cellular carcinoma cell) (CVCL_7927 )
Cell Originated Tissue	Liver
Infection Time	48 h
Interaction Score	FDR ≤ 0.05
Method Description	comprehensive identification of RNA-binding proteins by massspectrometry (ChIRP-MS)

Differential Gene Expression During SARS-COV-2 Infection

GEO Accession: GSE152641

Sample Type: Blood

Samples Details: Healthy Control: 24; COVID-19: 62

Platform: GPL24676 Illumina NovaSeq 6000

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GEO Accession: GSE162835

Sample Type: Nasopharyngeal Swabs

Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3

Platform: GPL24676 Illumina NovaSeq 6000

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GEO Accession: GSE175779

Sample Type: Human Bronchial Epithelial Cells

Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)

Platform: GPL18573 Illumina NextSeq 500

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Protein Sequence Information
MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI Click to Show/Hide

References
1	Omega-oxidation of 20-hydroxyeicosatetraenoic acid (20-HETE) in cerebral microvascular smooth muscle and endothelium by alcohol dehydrogenase 4. J Biol Chem. 2005 Sep 30;280(39):33157-64.
2	Mutation of Arg-115 of human class III alcohol dehydrogenase: a binding site required for formaldehyde dehydrogenase activity and fatty acid activation. Proc Natl Acad Sci USA. 1993 Mar 15;90(6):2491-4.
3	Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2. Nat Commun. 2022 Apr 25;13(1):2237.
4	Discovery and functional interrogation of SARS-CoV-2 RNA-host protein interactions. Cell. 2021 Apr 29;184(9):2394-2411.e16.