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ManualDetails of Host Protein
| Host Protein General Information (ID: PT0272) | |||||||||
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| Protein Name |
CPSF 73 kDa subunit (CPSF73)
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Gene Name |
CPSF3
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| Host Species |
Homo sapiens
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Uniprot Entry Name |
CPSF3_HUMAN
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| Protein Families |
Metallo-beta-lactamase superfamily
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| EC Number |
3.1.27.-
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| Subcellular Location |
Nucleus
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| External Link | |||||||||
| NCBI Gene ID | |||||||||
| Uniprot ID | |||||||||
| Ensembl ID | |||||||||
| HGNC ID | |||||||||
| Function in Host |
Component of the cleavage and polyadenylation specificityfactor (CPSF) complex that plays a key role in pre-mRNA 3'-endformation, recognizing the AAUAAA signal sequence and interacting withpoly (A) polymerase and other factors to bring about cleavage andpoly (A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in thehistone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavageof histone pre-mRNAs and acts as a 5' to 3' exonuclease for degradingthe subsequent downstream cleavage product (DCP) of mature histonemRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histonepre-mRNA leaving a 3'hydroxyl group on the upstream fragment containingthe stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3'exonuclease activity is processive and degrades the DCP RNA substrateeven after complete removal of the U7-binding site. Binds to thedownstream cleavage product (DCP) of histone pre-mRNAs and the cleavedDCP RNA substrate in a U7 snRNP dependent manner. Required forentering/progressing through S-phase of the cell cycle. Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interactionwith ISY1. Required for the biogenesis of all miRNAsfrom the pri-miR-17-92 primary transcript except miR-92a. Only required for the biogenesis of miR-290 and miR-96from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively.
[1-5]
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| Related KEGG Pathway | |||||||||
| mRNA surveillance pathway | hsa03015 |
Pathway Map 
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| 3D Structure |
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| Full List of Virus RNA Interacting with This Protien | |||||||||
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| RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain ) | |||||||||
| RNA Region Details |
RNA Info
Click to show the detail information of this RNA binding region
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[6] | |||||||
| Strains Name |
hCoV-19/Not Specified Virus Strain
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| RNA Binding Region |
ORF10
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| Virus Name |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
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| Infection Cells | Huh7 cells (human liver cell line) (CVCL_0336 ) | ||||||||
| Cell Originated Tissue | Liver | ||||||||
| Interaction Score | P-value < 0.05 | ||||||||
| Method Description | RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS) | ||||||||
| Differential Gene Expression During SARS-COV-2 Infection | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Protein Phosphorylation after Virus Infection | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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T681
[7] |
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T681
[8] |
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| Protein Sequence Information |
MSAIPAEESDQLLIRPLGAGQEVGRSCIILEFKGRKIMLDCGIHPGLEGMDALPYIDLIDPAEIDLLLISHFHLDHCGALPWFLQKTSFKGRTFMTHATKAIYRWLLSDYVKVSNISADDMLYTETDLEESMDKIETINFHEVKEVAGIKFWCYHAGHVLGAAMFMIEIAGVKLLYTGDFSRQEDRHLMAAEIPNIKPDILIIESTYGTHIHEKREEREARFCNTVHDIVNRGGRGLIPVFALGRAQELLLILDEYWQNHPELHDIPIYYASSLAKKCMAVYQTYVNAMNDKIRKQININNPFVFKHISNLKSMDHFDDIGPSVVMASPGMMQSGLSRELFESWCTDKRNGVIIAGYCVEGTLAKHIMSEPEEITTMSGQKLPLKMSVDYISFSAHTDYQQTSEFIRALKPPHVILVHGEQNEMARLKAALIREYEDNDEVHIEVHNPRNTEAVTLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNFNYHILSPCDLSNYTDLAMSTVKQTQAIPYTGPFNLLCYQLQKLTGDVEELEIQEKPALKVFKNITVIQEPGMVVLEWLANPSNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLEMHVYSKRLEIMLQDIFGEDCVSVKDDSILSVTVDGKTANLNLETRTVECEEGSEDDESLREMVELAAQRLYEALTPVH
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