Details of Host Protein

 Host Protein General Information (ID: PT0321)
  Protein Name
Cytosolic phospholipase A2 (GIVA cPLA2)
  Gene Name
PLA2G4A
  Host Species
Homo sapiens
  Uniprot Entry Name
PA24A_HUMAN
  EC Number
3.1.1.4; 3.1.1.5
  Subcellular Location
Cytoplasm and cytosol; nucleus
  External Link
NCBI Gene ID
5321
Uniprot ID
P47712
Ensembl ID
ENSG00000183914
HGNC ID
HGNC:9035
  Function in Host
Has primarily calcium-dependent phospholipase andlysophospholipase activities, with a major role in membrane lipidremodeling and biosynthesis of lipid mediators of the inflammatoryresponse. Plays an importantrole in embryo implantation and parturition through its ability totrigger prostanoid production. Preferentiallyhydrolyzes the ester bond of the fatty acyl group attached at sn-2position of phospholipids (phospholipase A2 activity). Selectively hydrolyzes sn-2 arachidonoyl group frommembrane phospholipids, providing the precursor for eicosanoidbiosynthesis via the cyclooxygenase pathway. Inan alternative pathway of eicosanoid biosynthesis, hydrolyzes sn-2fatty acyl chain of eicosanoid lysophopholipids to release freebioactive eicosanoids. Hydrolyzes the ester bond ofthe fatty acyl group attached at sn-1 position of phospholipids (phospholipase A1 activity) only if an ether linkage rather than anester linkage is present at the sn-2 position. This hydrolysis is notstereospecific. Has calcium-independent phospholipaseA2 and lysophospholipase activities in the presence ofphosphoinositides. Has O-acyltransferase activity. Catalyzes the transfer of fatty acyl chains from phospholipids to aprimary hydroxyl group of glycerol (sn-1 or sn-3), potentiallycontributing to monoacylglycerol synthesis. [1-10]
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  Related KEGG Pathway
Platelet activation hsa04611            Pathway Map 
Fc epsilon RI signaling pathway hsa04664            Pathway Map 
Fc gamma R-mediated phagocytosis hsa04666            Pathway Map 
MAPK signaling pathway hsa04010            Pathway Map 
Ras signaling pathway hsa04014            Pathway Map 
Phospholipase D signaling pathway hsa04072            Pathway Map 
Necroptosis hsa04217            Pathway Map 
VEGF signaling pathway hsa04370            Pathway Map 
  3D Structure
PDB ID: 1BCI 
FASTA   Download
  2D PNG Download  
PDB File Download

 Full List of Virus RNA Interacting with This Protien
            RNA Region: 3'-UTR (hCoV-19/Wuhan-Hu-1/2019 )
              RNA Region Details RNA Info Click to show the detail information of this RNA binding region [11]
              Strains Name
hCoV-19/Wuhan-Hu-1/2019
              Strains Family
Beta (B.1.351)
              RNA Binding Region
3'-UTR
              Virus Name
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
              Infection Cells HEK293 Cells (Human embryonic kidney cell)  (CVCL_0045 )
              Cell Originated Tissue Liver
              Infection Time 48 h
              Interaction Score Prot score = 53
              Method Description RNA-protein interaction detection (RaPID) assay; liquid chromatography with tandem mass spectrometry (LC-MS/MS)

Differential Gene Expression During SARS-COV-2 Infection
GEO Accession: GSE152641
Sample Type: Blood
Samples Details: Healthy Control: 24; COVID-19: 62
Platform: GPL24676 Illumina NovaSeq 6000
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GEO Accession: GSE162835
Sample Type: Nasopharyngeal Swabs
Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3
Platform: GPL24676 Illumina NovaSeq 6000
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GEO Accession: GSE175779
Sample Type: Human Bronchial Epithelial Cells
Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)
Platform: GPL18573 Illumina NextSeq 500
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Protein Phosphorylation after Virus Infection
S437 [12]
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S727 [12]
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S729 [12]
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T268 [12]
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Protein Sequence Information
MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDETLGTATFTVSSMKVGEKKEVPFIFNQVTEMVLEMSLEVCSCPDLRFSMALCDQEKTFRQQRKEHIRESMKKLLGPKNSEGLHSARDVPVVAILGSGGGFRAMVGFSGVMKALYESGILDCATYVAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLWKKKSSGQPVTFTDIFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELMFADWVEFSPYEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSRGSTMEEELENITTKHIVSNDSSDSDDESHEPKGTENEDAGSDYQSDNQASWIHRMIMALVSDSALFNTREGRAGKVHNFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDMEKDCPTIIHFVLANINFRKYRAPGVPRETEEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMHFNTLNNIDVIKEAMVESIEYRRQNPSRCSVSLSNVEARRFFNKEFLSKPKA
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References
1 Identification of essential residues for the catalytic function of 85-kDa cytosolic phospholipase A2 Probing the role of histidine, aspartic acid, cysteine, and arginine. J Biol Chem. 1996 Aug 9;271(32):19225-31.
2 Cyclooxygenase-2 Mediated Oxidation of 2-Arachidonoyl-Lysophospholipids Identifies Unknown Lipid Signaling Pathways. Cell Chem Biol. 2016 Oct 20;23(10):1217-1227.
3 Inherited human cPLA(2alpha) deficiency is associated with impaired eicosanoid biosynthesis, small intestinal ulceration, and platelet dysfunction. J Clin Invest. 2008 Jun;118(6):2121-31.
4 Ceramide-1-phosphate binds group IVA cytosolic phospholipase a2 via a novel site in the C2 domain. J Biol Chem. 2007 Jul 13;282(28):20467-74.
5 Identification of the expressed form of human cytosolic phospholipase A2beta (cPLA2beta): cPLA2beta3 is a novel variant localized to mitochondria and early endosomes. J Biol Chem. 2006 Jun 16;281(24):16615-24.
6 Cloning of a gene for a novel epithelium-specific cytosolic phospholipase A2, cPLA2delta, induced in psoriatic skin. J Biol Chem. 2004 Mar 26;279(13):12890-7.
7 Molecular characterization of cytosolic phospholipase A2-beta. J Biol Chem. 1999 Jun 11;274(24):17063-7.
8 Inhibition of human cytosolic phospholipase A2 by human annexin V. Biochem J. 1998 Jan 15;329 ( Pt 2)(Pt 2):369-72.
9 Functional identification of the active-site nucleophile of the human 85-kDa cytosolic phospholipase A2. Biochemistry. 1996 Mar 26;35(12):3712-21.
10 Multiple enzymatic activities of the human cytosolic 85-kDa phospholipase A2: hydrolytic reactions and acyl transfer to glycerol. Biochemistry. 1995 Jun 20;34(24):7807-18.
11 RNA-Protein Interaction Analysis of SARS-CoV-2 5 and 3 Untranslated Regions Reveals a Role of Lysosome-Associated Membrane Protein-2a during Viral Infection. mSystems. 2021 Aug 31;6(4):e0064321.
12 Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.