Details of Host Protein

 Host Protein General Information (ID: PT0348)
  Protein Name
DNA topoisomerase II alpha (TOP2A)
  Gene Name
TOP2A
  Host Species
Homo sapiens
  Uniprot Entry Name
TOP2A_HUMAN
  Protein Families
Type II topoisomerase family
  EC Number
5.6.2.2
  Subcellular Location
Cytoplasm Nucleus; nucleoplasm Nucleus; nucleolus
  External Link
NCBI Gene ID
7153
Uniprot ID
P11388
Ensembl ID
ENSG00000131747
HGNC ID
HGNC:11989
  Function in Host
Key decatenating enzyme that alters DNA topology by bindingto two double-stranded DNA molecules, generating a double-strandedbreak in one of the strands, passing the intact strand through thebroken strand, and religating the broken strand. May play a role inregulating the period length of ARNTL/BMAL1 transcriptional oscillation. [1-4]
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  Related KEGG Pathway
Platinum drug resistance hsa01524            Pathway Map 
  3D Structure
PDB ID: 1ZXM 
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  2D PNG Download  
PDB File Download

 Full List of Virus RNA Interacting with This Protien
            RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain )
              RNA Region Details RNA Info Click to show the detail information of this RNA binding region [5]
              Strains Name
hCoV-19/Not Specified Virus Strain
              RNA Binding Region
ORF10
              Virus Name
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
              Infection Cells Calu-3 cells (Human Lung Cancer Cell)  (CVCL_0609 )
              Cell Originated Tissue Liver
              Interaction Score P-value < 0.05
              Method Description RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS)

Differential Gene Expression During SARS-COV-2 Infection
GEO Accession: GSE152641
Sample Type: Blood
Samples Details: Healthy Control: 24; COVID-19: 62
Platform: GPL24676 Illumina NovaSeq 6000
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GEO Accession: GSE162835
Sample Type: Nasopharyngeal Swabs
Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3
Platform: GPL24676 Illumina NovaSeq 6000
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GEO Accession: GSE175779
Sample Type: Human Bronchial Epithelial Cells
Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)
Platform: GPL18573 Illumina NextSeq 500
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Protein Phosphorylation after Virus Infection
S1106 [6]
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S1213 [7]
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S1213 [6]
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S1247 [7]
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S1247 [6]
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S1377 [6]
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S1391 [6]
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S1392 [6]
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S1393 [6]
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S1449 [6]
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S1469 [6]
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S1525 [7]
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T1397 [6]
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Potential Drug(s) that Targets This Protein
Drug Name DrunkBank ID Pubchem ID TTD ID REF
Adriacin DB00997  443939  D07VLY  [8]
Adriacin DB00997  31703  D07VLY  [8]
AMSACRINE DB00276  2179  D0G9YH  [8]
CIPROFLOXACIN DB00537  2764  D0O1WX  [8]
DACTINOMYCIN DB00970  457193  D0P8IV  [8]
DAUNORUBICIN DB00694  30323  D01XWG  [8]
DAUNORUBICIN DB00694  9961878  . [8]
DAUNORUBICIN DB00694  62770  D01XWG  [8]
DEXRAZOXANE DB00380  71384  D07XVN  [8]
DIGITOXIN DB01396  441207  D0M3QP  [8]
ENOXACIN DB00467  3229  D0R8ER  [8]
EPIRUBICIN DB00445  41867  D0C9XJ  [8]
ETOPOSIDE DB00773  36462  D0B7EB  [8]
FINAFLOXACIN DB09047  11567473  D01IXD  [8]
FLUOROURACIL DB00544  3385  D05LEO  [8]
HYDROQUINONE DB09526  785  D03UOT  [8]
Idarubicin HCl DB01177  636362  D01XDL  [7]
LOMEFLOXACIN DB00978  3948  D02KOF  [8]
METHOTREXATE DB00563  126941  D0SV8E  [8]
MITOXANTRONE DB01204  4212  D0R3JB  [8]
MITOXANTRONE DB01204  51082  D0R3JB  [8]
MOXIFLOXACIN DB00218  152946  D0ZV0Z  [8]
NORFLOXACIN DB01059  4539  D0Q2PE  [8]
OFLOXACIN DB01165  4583  D03NHW  [8]
PACLITAXEL DB01229  36314  D01WYV  [8]
PEFLOXACIN DB00487  51081  D04NXQ  [8]
PODOFILOX DB01179  10607  D0D4HN  [8]
TENIPOSIDE DB00444  452548  D01DBQ  [8]
Triparanol . . . [8]
Triparanol . . . [8]
Triparanol . 6918092  . [8]
Triparanol . . . [8]
Triparanol . . . [8]
VINCRISTINE DB00541  5978  D09QVV  [8]

Protein Sequence Information
MEVSPLQPVNENMQVNKIKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNSLKVIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSGPTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAVEAKEKQDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF
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References
1 DNA cleavage and opening reactions of human topoisomerase IIa are regulated via Mg2+-mediated dynamic bending of gate-DNA. Proc Natl Acad Sci USA. 2012 Feb 21;109(8):2925-30.
2 RECQL5 cooperates with Topoisomerase II alpha in DNA decatenation and cell cycle progression. Nucleic Acids Res. 2012 Feb;40(4):1621-35.
3 The SET and transposase domain protein Metnase enhances chromosome decatenation: regulation by automethylation. Nucleic Acids Res. 2008 Oct;36(18):5822-31.
4 Nuclear interactions of topoisomerase II alpha and beta with phospholipid scramblase 1. Nucleic Acids Res. 2007;35(12):4076-85.
5 Mapping the host protein interactome of non-coding regions in SARS-CoV-2 genome. bioRxiv. 2021 Jun; DOI:10.1101/2021.06.19.449092.
6 Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.
7 The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell. 2020 Aug 6;182(3):685-712.e19.
8 Drug repurposing for coronavirus (SARS-CoV-2) based on gene co-expression network analysis. Sci Rep. 2021 Nov 8;11(1):21872.