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ManualDetails of Host Protein
| Host Protein General Information (ID: PT0360) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Protein Name |
DDOST 48 kDa subunit (APOBEC3C)
|
Gene Name |
DDOST
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| Host Species |
Homo sapiens
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Uniprot Entry Name |
OST48_HUMAN
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| Protein Families |
DDOST 48 kDa subunit family
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| Subcellular Location |
Endoplasmic reticulum
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| External Link | |||||||||
| NCBI Gene ID | |||||||||
| Uniprot ID | |||||||||
| Ensembl ID | |||||||||
| HGNC ID | |||||||||
| Function in Host |
Subunit of the oligosaccharyl transferase (OST) complex thatcatalyzes the initial transfer of a defined glycan (Glc (3) Man (9) GlcNAc (2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thrconsensus motif in nascent polypeptide chains, the first step inprotein N-glycosylation. N-glycosylation occurscotranslationally and the complex associates with the Sec61 complex atthe channel-forming translocon complex that mediates proteintranslocation across the endoplasmic reticulum (ER). All subunits arerequired for a maximal enzyme activity. Required forthe assembly of both SST3A- and SS3B-containing OST complexes.
[1-2]
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| Related KEGG Pathway | |||||||||
| Protein processing in endoplasmic reticulum | hsa04141 |
Pathway Map 
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| Metabolic pathways | hsa01100 |
Pathway Map 
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| N-Glycan biosynthesis | hsa00510 |
Pathway Map 
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| Various types of N-glycan biosynthesis | hsa00513 |
Pathway Map 
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| 3D Structure |
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| Function of This Protein During Virus Infection | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Virus Name | SARS-COV-2 | Protein Function | Anti-viral | [3] | |||||
| Infected Tissue | Lung | Infection Time | 7-9 Days | ||||||
| Infected Cell | Calu-3 Cells (Human epithelial cell line) | Cellosaurus ID | CVCL_0609 | ||||||
| Method Description | To detect the role of host protein DDOST in viral infection, DDOST protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen. | ||||||||
| Results | It is reported that knockout of DDOST increases SARS-CoV-2 RNA levels compared with control group. | ||||||||
| Full List of Virus RNA Interacting with This Protien | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain ) | |||||||||
| RNA Region Details |
RNA Info
Click to show the detail information of this RNA binding region
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[4] | |||||||
| Strains Name |
hCoV-19/Not Specified Virus Strain
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| RNA Binding Region |
ORF10
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| Virus Name |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
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| Infection Cells | Huh7 cells (human liver cell line) (CVCL_0336 ) | ||||||||
| Cell Originated Tissue | Liver | ||||||||
| Interaction Score | P-value < 0.05 | ||||||||
| Method Description | RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS) | ||||||||
