Details of Host Protein

KEGG: hsa00010 Pathway Download ×

KEGG: hsa00030 Pathway Download ×

KEGG: hsa00500 Pathway Download ×

KEGG: hsa00520 Pathway Download ×

KEGG: hsa01100 Pathway Download ×

KEGG: hsa01200 Pathway Download ×

KEGG: hsa01250 Pathway Download ×

Host Protein General Information (ID: PT0472)
Protein Name	Glucose-6-phosphate isomerase (GPI)	Gene Name	GPI
Host Species	Homo sapiens	Uniprot Entry Name	G6PI_HUMAN
Protein Families	GPI family
EC Number	5.3.1.9
Subcellular Location	Cytoplasm Secreted
External Link
	NCBI Gene ID	2821
	Uniprot ID	P06744
	Ensembl ID	ENSG00000105220
	HGNC ID	HGNC:4458
Function in Host	In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, andthe reverse reaction during gluconeogenesis. Besidesit's role as a glycolytic enzyme, also acts as a secreted cytokine:acts as an angiogenic factor (AMF) that stimulates endothelial cellmotility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It issecreted by lectin-stimulated T-cells and induces immunoglobulinsecretion. [1-4] Click to Show/Hide
Related KEGG Pathway
	Glycolysis / Gluconeogenesis	hsa00010		Pathway Map
	Pentose phosphate pathway	hsa00030		Pathway Map
	Starch and sucrose metabolism	hsa00500		Pathway Map
	Amino sugar and nucleotide sugar metabolism	hsa00520		Pathway Map
	Metabolic pathways	hsa01100		Pathway Map
	Carbon metabolism	hsa01200		Pathway Map
	Biosynthesis of nucleotide sugars	hsa01250		Pathway Map
3D Structure	PDB ID: 1IAT FASTA Download 2D PNG Download PDB File Download

Function of This Protein During Virus Infection
Virus Name	SARS-COV-2	Protein Function	Pro-viral	[5]
Infected Tissue	Lung	Infection Time	24 h
Infected Cell	A549 Cells (Adenocarcinomic Human alveolar basal epithelial cell)	Cellosaurus ID	CVCL_H249
Method Description	To detect the role of host protein GPI in viral infection, GPI protein knockout A549 Cells were infected with SARS-COV-2 for 24 h , and the effects on infection was detected through qRT-PCR.
Results	It is reported that knockdown of GPI leads to the reduced viral particles production 50% compared with control group.

Host Protein - Virus RNA Network

Network Download

Full List of Virus RNA Interacting with This Protien
*RNA Region: Not Specified Virus Region (hCoV-19/USA/WA1/2020 )*
RNA Region Details	RNA Info Click to show the detail information of this RNA binding region	[6]
Strains Name	hCoV-19/USA/WA1/2020
Strains Family	Alpha (B.1.1.7)
RNA Binding Region	Not Specified Virus Region
Virus Name	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Infection Cells	Huh7.5 cells (Hepatocyte derived cellular carcinoma cell) (CVCL_7927 )
Cell Originated Tissue	Liver
Infection Time	48 h
Interaction Score	FDR ≤ 0.05
Method Description	comprehensive identification of RNA-binding proteins by massspectrometry (ChIRP-MS)
*RNA Region: Not Specified Virus Region (hCoV-19/France/IDF-220-95/2020 )*
RNA Region Details	RNA Info Click to show the detail information of this RNA binding region	[5]
Strains Name	hCoV-19/France/IDF-220-95/2020
RNA Binding Region	Not Specified Virus Region
Virus Name	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Interaction Type	Direct interaction
Infection Cells	HEK293 Cells (Human embryonic kidney cell) HEK293 Cells (Human embryonic kidney cell) (CVCL_0045 )
Cell Originated Tissue	Kidney
Infection Time	48 h
Interaction Score	SAINT score ≥ 0.79
Method Description	comprehensive identification of RNA-binding proteins by massspectrometry (ChIRP-MS)

Differential Gene Expression During SARS-COV-2 Infection

GEO Accession: GSE152641

Sample Type: Blood

Samples Details: Healthy Control: 24; COVID-19: 62

Platform: GPL24676 Illumina NovaSeq 6000

PNG Download

GEO Accession: GSE162835

Sample Type: Nasopharyngeal Swabs

Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3

Platform: GPL24676 Illumina NovaSeq 6000

PNG Download

GEO Accession: GSE175779

Sample Type: Human Bronchial Epithelial Cells

Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)

Platform: GPL18573 Illumina NextSeq 500

PNG Download

Protein Phosphorylation after Virus Infection
N86 [7] PNG Download
S107 [8] PNG Download
S22 [7] PNG Download
T109 [8] PNG Download

Protein Sequence Information
MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDNFEQLLSGAHWMDQHFRTTPLEKNAPVLLALLGIWYINCFGCETHAMLPYDQYLHRFAAYFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMRGKSTEEARKELQAAGKSPEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGALVAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSAQVTSHDASTNGLINFIKQQREARVQ Click to Show/Hide

References
1	Mouse glucose-6-phosphate isomerase and neuroleukin have identical 3 sequences. Nature. 1988 Mar 31;332(6163):455-7.
2	Two novel mutations (p.(Ser160Pro) and p.(Arg472Cys)) causing glucose-6-phosphate isomerase deficiency are associated with erythroid dysplasia and inappropriately suppressed hepcidin. Blood Cells Mol Dis. 2018 Mar;69:23-29.
3	Tumor autocrine motility factor is an angiogenic factor that stimulates endothelial cell motility. Biochem Biophys Res Commun. 2001 Jul 6;285(1):118-28.
4	Phosphohexose isomerase/autocrine motility factor/neuroleukin/maturation factor is a multifunctional phosphoprotein. Biochim Biophys Acta. 2000 Jul 14;1480(1-2):235-44.
5	Characterization and functional interrogation of the SARS-CoV-2 RNA interactome. Cell Rep. 2022 Apr 26;39(4):110744.
6	Discovery and functional interrogation of SARS-CoV-2 RNA-host protein interactions. Cell. 2021 Apr 29;184(9):2394-2411.e16.
7	The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell. 2020 Aug 6;182(3):685-712.e19.
8	Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.