Details of Host Protein

Host Protein General Information (ID: PT0593)
Protein Name	Kinesin-like protein KIF13B (SREBF2)	Gene Name	KIF13B
Host Species	Homo sapiens	Uniprot Entry Name	KI13B_HUMAN
Protein Families	TRAFAC class myosin-kinesin ATPase superfamily
Subcellular Location	Cytoplasm; cytoskeleton Cell projection; axon
External Link
	NCBI Gene ID	23303
	Uniprot ID	Q9NQT8
	Ensembl ID	ENSG00000198911
	HGNC ID	HGNC:14405
Function in Host	Involved in reorganization of the cortical cytoskeleton. Regulates axon formation by promoting the formation of extra axons. Maybe functionally important for the intracellular trafficking of MAGUKsand associated protein complexes. Click to Show/Hide
3D Structure	PDB ID: 2COW FASTA Download 2D PNG Download PDB File Download

Function of This Protein During Virus Infection
Virus Name	HCoV-229E	Protein Function	Pro-viral	[1]
Infected Tissue	Liver	Infection Time	4 days
Infected Cell	Huh-7.5 Cells (Human hepatocellular carcinoma cells)	Cellosaurus ID	CVCL_7927
Method Description	To detect the role of host protein KIF13B in viral infection, KIF13B protein knockout Huh-7.5 Cells were infected with HCoV-229E for 4 days , and the effects on infection was detected through kit.
Results	It is reported that Knockout of KIF13B leads to the disruption appeared to block infection compared with control group.
Virus Name	HCoV-NL63	Protein Function	Pro-viral	[2]
Infected Tissue	Liver	Infection Time	24 h
Infected Cell	Huh-7.5 Cells (Human hepatocellular carcinoma cells)	Cellosaurus ID	CVCL_7927
Method Description	To detect the role of host protein KIF13B in viral infection, KIF13B protein knockout Huh-7.5 Cells were infected with HCoV-NL63 for 24 h , and the effects on infection was detected through qRT-PCR.
Results	It is reported that knockdown of KIF13B leads to the reduced SARS-CoV-2 RNA levels compared with control group.
Virus Name	HCoV-OC43	Protein Function	Pro-viral	[2]
Infected Tissue	Liver	Infection Time	24 h
Infected Cell	Huh-7.5 Cells (Human hepatocellular carcinoma cells)	Cellosaurus ID	CVCL_7927
Method Description	To detect the role of host protein KIF13B in viral infection, KIF13B protein knockout Huh-7.5 Cells were infected with HCoV-OC43 for 24 h , and the effects on infection was detected through qRT-PCR.
Results	It is reported that knockdown of KIF13B leads to the reduced SARS-CoV-2 RNA levels compared with control group.
Virus Name	HCoV-OC43	Protein Function	Pro-viral	[1]
Infected Tissue	Liver	Infection Time	4 days
Infected Cell	Huh-7.5 Cells (Human hepatocellular carcinoma cells)	Cellosaurus ID	CVCL_7927
Method Description	To detect the role of host protein KIF13B in viral infection, KIF13B protein knockout Huh-7.5 Cells were infected with HCoV-OC43 for 4 days , and the effects on infection was detected through kit.
Results	It is reported that Knockout of KIF13B leads to the disruption appeared to block infection compared with control group.
Virus Name	SARS-COV-2	Protein Function	Pro-viral	[2]
Infected Tissue	Liver	Infection Time	24 h
Infected Cell	Huh-7.5 Cells (Human hepatocellular carcinoma cells)	Cellosaurus ID	CVCL_7927
Method Description	To detect the role of host protein KIF13B in viral infection, KIF13B protein knockout Huh-7.5 Cells were infected with SARS-COV-2 for 24 h , and the effects on infection was detected through qRT-PCR.
Results	It is reported that knockdown of KIF13B leads to the reduced SARS-CoV-2 RNA levels compared with control group.
Virus Name	SARS-COV-2	Protein Function	Pro-viral	[3]
Infected Tissue	Lung	Infection Time	7-9 Days
Infected Cell	Calu-3 Cells (Human epithelial cell line)	Cellosaurus ID	CVCL_0609
Method Description	To detect the role of host protein KIF13B in viral infection, KIF13B protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen.
Results	It is reported that knockout of KIF13B leads to the decreased SARS-CoV-2 RNA levels compared with control group.
Virus Name	SARS-COV-2	Protein Function	Pro-viral	[3]
Infected Tissue	Lung	Infection Time	7-9 Days
Infected Cell	Calu-3 Cells (Human epithelial cell line)	Cellosaurus ID	CVCL_0609
Method Description	To detect the role of host protein KIF13B in viral infection, KIF13B protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen.
Results	It is reported that knockout of KIF13B leads to the decreased SARS-CoV-2 RNA levels compared with control group.

Full List of Virus RNA Interacting with This Protien
*RNA Region: 5'-UTR (hCoV-19/Wuhan-Hu-1/2019 )*
RNA Region Details	RNA Info Click to show the detail information of this RNA binding region	[4]
Strains Name	hCoV-19/Wuhan-Hu-1/2019
Strains Family	Beta (B.1.351)
RNA Binding Region	5'-UTR
Virus Name	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Infection Cells	HEK293 Cells (Human embryonic kidney cell) (CVCL_0045 )
Cell Originated Tissue	Liver
Infection Time	48 h
Interaction Score	Prot score = 30
Method Description	RNA-protein interaction detection (RaPID) assay; liquid chromatography with tandem mass spectrometry (LC-MS/MS)

Differential Gene Expression During SARS-COV-2 Infection

GEO Accession: GSE152641

Sample Type: Blood

Samples Details: Healthy Control: 24; COVID-19: 62

Platform: GPL24676 Illumina NovaSeq 6000

PNG Download

GEO Accession: GSE162835

Sample Type: Nasopharyngeal Swabs

Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3

Platform: GPL24676 Illumina NovaSeq 6000

PNG Download

GEO Accession: GSE175779

Sample Type: Human Bronchial Epithelial Cells

Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)

Platform: GPL18573 Illumina NextSeq 500

PNG Download

Protein Phosphorylation after Virus Infection
S1381 [5] PNG Download
S1381 [6] PNG Download
S1382 [6] PNG Download
S1389 [6] PNG Download
S1391 [6] PNG Download
S1410 [5] PNG Download
S1410 [6] PNG Download
S1497 [6] PNG Download
S1644 [6] PNG Download
S1797 [5] PNG Download
S645 [5] PNG Download
S661 [5] PNG Download
S661 [6] PNG Download
T1799 [6] PNG Download

Protein Sequence Information
MGDSKVKVAVRIRPMNRRETDLHTKCVVDVDANKVILNPVNTNLSKGDARGQPKVFAYDHCFWSMDESVKEKYAGQDIVFKCLGENILQNAFDGYNACIFAYGQTGSGKSYTMMGTADQPGLIPRLCSGLFERTQKEENEEQSFKVEVSYMEIYNEKVRDLLDPKGSRQTLKVREHSVLGPYVDGLSKLAVTSYKDIESLMSEGNKSRTVAATNMNEESSRSHAVFKITLTHTLYDVKSGTSGEKVGKLSLVDLAGSERATKTGAAGDRLKEGSNINKSLTTLGLVISALADQSAGKNKNKFVPYRDSVLTWLLKDSLGGNSKTAMVATVSPAADNYDETLSTLRYADRAKHIVNHAVVNEDPNARIIRDLREEVEKLREQLTKAEAMKSPELKDRLEESEKLIQEMTVTWEEKLRKTEEIAQERQKQLESLGISLQSSGIKVGDDKCFLVNLNADPALNELLVYYLKEHTLIGSANSQDIQLCGMGILPEHCIIDITSEGQVMLTPQKNTRTFVNGSSVSSPIQLHHGDRILWGNNHFFRLNLPKKKKKAEREDEDQDPSMKNENSSEQLDVDGDSSSEVSSEVNFNYEYAQMEVTMKALGSNDPMQSILNSLEQQHEEEKRSALERQRLMYEHELEQLRRRLSPEKQNCRSMDRFSFHSPSAQQRLRQWAEEREATLNNSLMRLREQIVKANLLVREANYIAEELDKRTEYKVTLQIPASSLDANRKRGSLLSEPAIQVRRKGKGKQIWSLEKLDNRLLDMRDLYQEWKECEEDNPVIRSYFKRADPFYDEQENHSLIGVANVFLESLFYDVKLQYAVPIINQKGEVAGRLHVEVMRLSGDVGERIAGGDEVAEVSFEKETQENKLVCMVKILQATGLPQHLSHFVFCKYSFWDQQEPVIVAPEVDTSSSSVSKEPHCMVVFDHCNEFSVNITEDFIEHLSEGALAIEVYGHKINDPRKNPALWDLGIIQAKTRSLRDRWSEVTRKLEFWVQILEQNENGEYCPVEVISAKDVPTGGIFQLRQGQSRRVQVEVKSVQESGTLPLMEECILSVGIGCVKVRPLRAPRTHETFHEEEEDMDSYQDRDLERLRRKWLNALTKRQEYLDQQLQKLVSKRDKTEDDADREAQLLEMRLTLTEERNAVMVPSAGSGIPGAPAEWTPVPGMETHIPVIFLDLNADDFSSQDNLDDPEAGGWDATLTGEEEEEFFELQIVKQHDGEVKAEASWDSAVHGCPQLSRGTPVDERLFLIVRVTVQLSHPADMQLVLRKRICVNVHGRQGFAQSLLKKMSHRSSIPGCGVTFEIVSNIPEDAQGVEEREALARMAANVENPASADSEAYIEKYLRSVLAVENLLTLDRLRQEVAVKEQLTGKGKLSRRSISSPNVNRLSGSRQDLIPSYSLGSNKGRWESQQDVSQTTVSRGIAPAPALSVSPQNNHSPDPGLSNLAASYLNPVKSFVPQMPKLLKSLFPVRDEKRGKRPSPLAHQPVPRIMVQSASPDIRVTRMEEAQPEMGPDVLVQTMGAPALKICDKPAKVPSPPPVIAVTAVTPAPEAQDGPPSPLSEASSGYFSHSVSTATLSDALGPGLDAAAPPGSMPTAPEAEPEAPISHPPPPTAVPAEEPPGPQQLVSPGRERPDLEAPAPGSPFRVRRVRASELRSFSRMLAGDPGCSPGAEGNAPAPGAGGQALASDSEEADEVPEWLREGEFVTVGAHKTGVVRYVGPADFQEGTWVGVELDLPSGKNDGSIGGKQYFRCNPGYGLLVRPSRVRRATGPVRRRSTGLRLGAPEARRSATLSGSATNLASLTAALAKADRSHKNPENRKSWAS Click to Show/Hide

References
1	Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks. Cell. 2021 Jan 7;184(1):120-132.e14.
2	Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors. Cell Host Microbe. 2021 Feb 10;29(2):267-280.e5.
3	Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2. Nat Commun. 2022 Apr 25;13(1):2237.
4	RNA-Protein Interaction Analysis of SARS-CoV-2 5 and 3 Untranslated Regions Reveals a Role of Lysosome-Associated Membrane Protein-2a during Viral Infection. mSystems. 2021 Aug 31;6(4):e0064321.
5	The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell. 2020 Aug 6;182(3):685-712.e19.
6	Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.

Details of Host Protein

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