Details of Host Protein

 Host Protein General Information (ID: PT0667)
  Protein Name
Ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50)
  Gene Name
SLC9A3R1
  Host Species
Homo sapiens
  Uniprot Entry Name
NHRF1_HUMAN
  Subcellular Location
Cytoplasm Apical cell membrane Endomembrane system
  External Link
NCBI Gene ID
9368
Uniprot ID
O14745
Ensembl ID
ENSG00000109062
HGNC ID
HGNC:11075
  Function in Host
Scaffold protein that connects plasma membrane proteins withmembers of the ezrin/moesin/radixin family and thereby helps to linkthem to the actin cytoskeleton and to regulate their surfaceexpression. Necessary for recycling of internalized ADRB2. Was firstknown to play a role in the regulation of the activity and subcellularlocation of SLC9A3. Necessary for cAMP-mediated phosphorylation andinhibition of SLC9A3. May enhance Wnt signaling. May participate inHTR4 targeting to microvilli. Involved in theregulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation ofthe chloride and bicarbonate homeostasis in spermatozoa.
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  Related KEGG Pathway
Human papillomavirus infection hsa05165            Pathway Map 
Pathogenic Escherichia coli infection hsa05130            Pathway Map 
Tight junction hsa04530            Pathway Map 
Parathyroid hormone synthesis, secretion and action hsa04928            Pathway Map 
  3D Structure
PDB ID: 1G9O 
FASTA   Download
  2D PNG Download  
PDB File Download

Function of This Protein During Virus Infection
Virus NameSARS-COV-2 Protein Function Anti-viral [1]
Infected TissueLung Infection Time7-9 Days
Infected CellCalu-3 Cells (Human epithelial cell line) Cellosaurus IDCVCL_0609 
Method DescriptionTo detect the role of host protein SLC9A3R1 in viral infection, SLC9A3R1 protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen.
ResultsIt is reported that knockout of SLC9A3R1 increases SARS-CoV-2 RNA levels compared with control group.

 Full List of Virus RNA Interacting with This Protien
            RNA Region: Not Specified Virus Region (hCoV-19/USA/WA1/2020 )
              RNA Region Details RNA Info Click to show the detail information of this RNA binding region [2]
              Strains Name
hCoV-19/USA/WA1/2020
              Strains Family
Alpha (B.1.1.7)
              RNA Binding Region
Not Specified Virus Region
              Virus Name
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
              Infection Cells Huh7.5 cells (Hepatocyte derived cellular carcinoma cell)  (CVCL_7927 )
              Cell Originated Tissue Liver
              Infection Time 48 h
              Interaction Score FDR ≤ 0.05
              Method Description comprehensive identification of RNA-binding proteins by massspectrometry (ChIRP-MS)

Differential Gene Expression During SARS-COV-2 Infection
GEO Accession: GSE152641
Sample Type: Blood
Samples Details: Healthy Control: 24; COVID-19: 62
Platform: GPL24676 Illumina NovaSeq 6000
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GEO Accession: GSE162835
Sample Type: Nasopharyngeal Swabs
Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3
Platform: GPL24676 Illumina NovaSeq 6000
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GEO Accession: GSE175779
Sample Type: Human Bronchial Epithelial Cells
Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)
Platform: GPL18573 Illumina NextSeq 500
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Protein Phosphorylation after Virus Infection
S280 [3]
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S280 [4]
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S288 [4]
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S290 [4]
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T293 [4]
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Protein Sequence Information
MSADAAAGAPLPRLCCLEKGPNGYGFHLHGEKGKLGQYIRLVEPGSPAEKAGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLQKLGVQVREELLRAQEAPGQAEPPAAAEVQGAGNENEPREADKSHPEQRELRPRLCTMKKGPSGYGFNLHSDKSKPGQFIRSVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL
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References
1 Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2. Nat Commun. 2022 Apr 25;13(1):2237.
2 Discovery and functional interrogation of SARS-CoV-2 RNA-host protein interactions. Cell. 2021 Apr 29;184(9):2394-2411.e16.
3 The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell. 2020 Aug 6;182(3):685-712.e19.
4 Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.