Details of Host Protein

KEGG: hsa05164 Pathway Download ×

KEGG: hsa05168 Pathway Download ×

KEGG: hsa03008 Pathway Download ×

KEGG: hsa03013 Pathway Download ×

KEGG: hsa03015 Pathway Download ×

KEGG: hsa05014 Pathway Download ×

Host Protein General Information (ID: PT0689)
Protein Name	Nuclear RNA export factor 1 (NXF1)	Gene Name	NXF1
Host Species	Homo sapiens	Uniprot Entry Name	NXF1_HUMAN
Protein Families	NXF family
Subcellular Location	Nucleus
External Link
	NCBI Gene ID	10482
	Uniprot ID	Q9UBU9
	Ensembl ID	ENSG00000162231
	HGNC ID	HGNC:8071
Function in Host	Involved in the nuclear export of mRNA species bearingretroviral constitutive transport elements (CTE) and in the export ofmRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the exportof HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components ofthe TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1heterodimer for export. Also involved in nuclear export of m6A-containingmRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containingmRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. [1-5] Click to Show/Hide
Related KEGG Pathway
	Influenza A	hsa05164		Pathway Map
	Herpes simplex virus 1 infection	hsa05168		Pathway Map
	Ribosome biogenesis in eukaryotes	hsa03008		Pathway Map
	Nucleocytoplasmic transport	hsa03013		Pathway Map
	mRNA surveillance pathway	hsa03015		Pathway Map
	Amyotrophic lateral sclerosis	hsa05014		Pathway Map
3D Structure	PDB ID: 1FO1 FASTA Download 2D PNG Download PDB File Download

Function of This Protein During Virus Infection
Virus Name	SARS-COV-2	Protein Function	Pro-viral	[6]
Infected Tissue	Lung	Infection Time	7-9 Days
Infected Cell	Calu-3 Cells (Human epithelial cell line)	Cellosaurus ID	CVCL_0609
Method Description	To detect the role of host protein NXF1 in viral infection, NXF1 protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen.
Results	It is reported that knockout of NXF1 leads to the decreased SARS-CoV-2 RNA levels compared with control group.

Full List of Virus RNA Interacting with This Protien
*RNA Region: Not Specified Virus Region (hCoV-19/England/02/2020 )*
RNA Region Details	RNA Info Click to show the detail information of this RNA binding region	[7]
Strains Name	hCoV-19/England/02/2020
Strains Family	Beta (B.1.351)
RNA Binding Region	Not Specified Virus Region
Virus Name	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Infection Cells	Calu-3 cells (Human lung cancer cell) (CVCL_0609 )
Cell Originated Tissue	Lung
Infection Time	24 h
Interaction Score	P-adjust = 0.002
Method Description	UV protein-RNA crosslinking; RNA interactome capture (cRIC); RNA antisense purification coupled with mass spectrometry (RAP-MS)

Differential Gene Expression During SARS-COV-2 Infection

GEO Accession: GSE152641

Sample Type: Blood

Samples Details: Healthy Control: 24; COVID-19: 62

Platform: GPL24676 Illumina NovaSeq 6000

PNG Download

GEO Accession: GSE162835

Sample Type: Nasopharyngeal Swabs

Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3

Platform: GPL24676 Illumina NovaSeq 6000

PNG Download

GEO Accession: GSE175779

Sample Type: Human Bronchial Epithelial Cells

Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)

Platform: GPL18573 Illumina NextSeq 500

PNG Download

Protein Phosphorylation after Virus Infection
S9 [8] PNG Download

Protein Sequence Information
MADEGKSYSEHDDERVNFPQRKKKGRGPFRWKYGEGNRRSGRGGSGIRSSRLEEDDGDVAMSDAQDGPRVRYNPYTTRPNRRGDTWHDRDRIHVTVRRDRAPPERGGAGTSQDGTSKNWFKITIPYGRKYDKAWLLSMIQSKCSVPFTPIEFHYENTRAQFFVEDASTASALKAVNYKILDRENRRISIIINSSAPPHTILNELKPEQVEQLKLIMSKRYDGSQQALDLKGLRSDPDLVAQNIDVVLNRRSCMAATLRIIEENIPELLSLNLSNNRLYRLDDMSSIVQKAPNLKILNLSGNELKSERELDKIKGLKLEELWLDGNSLCDTFRDQSTYISAIRERFPKLLRLDGHELPPPIAFDVEAPTTLPPCKGSYFGTENLKSLVLHFLQQYYAIYDSGDRQGLLDAYHDGACCSLSIPFIPQNPARSSLAEYFKDSRNVKKLKDPTLRFRLLKHTRLNVVAFLNELPKTQHDVNSFVVDISAQTSTLLCFSVNGVFKEVDGKSRDSLRAFTRTFIAVPASNSGLCIVNDELFVRNASSEEIQRAFAMPAPTPSSSPVPTLSPEQQEMLQAFSTQSGMNLEWSQKCLQDNNWDYTRSAQAFTHLKAKGEIPEVAFMK Click to Show/Hide

References
1	YTHDC1 mediates nuclear export of N 6-methyladenosine methylated mRNAs. Elife. 2017 Oct 6;6:e31311.
2	Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA. EMBO J. 2009 Mar 4;28(5):556-67.
3	Mutually exclusive interactions drive handover of mRNA from export adaptors to TAP. Proc Natl Acad Sci USA. 2008 Apr 1;105(13):5154-9.
4	Specific interaction between RNA helicase A and Tap, two cellular proteins that bind to the constitutive transport element of type D retrovirus. J Biol Chem. 2000 Oct 20;275(42):32694-700.
5	TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus. Mol Cell. 1998 Apr;1(5):649-59.
6	Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2. Nat Commun. 2022 Apr 25;13(1):2237.
7	Global analysis of protein-RNA interactions in SARS-CoV-2-infected cells reveals key regulators of infection. Mol Cell. 2021 Jul 1;81(13):2851-2867.e7.
8	Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.