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ManualDetails of Host Protein
| Host Protein General Information (ID: PT0731) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Protein Name |
Splicing factor 3B-associated 14 kDa protein (SF3b14b)
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Gene Name |
PHF5A
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| Host Species |
Homo sapiens
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Uniprot Entry Name |
PHF5A_HUMAN
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| Protein Families |
PHF5 family
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| Subcellular Location |
Nucleus speckle
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| External Link | |||||||||
| NCBI Gene ID | |||||||||
| Uniprot ID | |||||||||
| Ensembl ID | |||||||||
| HGNC ID | |||||||||
| Function in Host |
Involved with the PAF1 complex (PAF1C) in transcriptionalelongation by RNA polymerase II, and in regulation of development andmaintenance of embryonic stem cell (ESC) pluripotency. Required formaintenance of ESCs self-renewal and cellular reprogramming of stemcells. Maintains pluripotency by recruiting and stabilizing PAF1C onpluripotency genes loci, and by regulating the expression of thepluripotency genes. Regulates the deposition of elongation-associatedhistone modifications, including dimethylated histone H3 'Lys-79' (H3K79me2) and trimethylated histone H3 'Lys-36' (H3K36me3), on PAF1Ctargets, self-renewal and pluripotency genes. Regulates RNA polymeraseII promoter-proximal pause release of the PAF1C targets and self-renewal genes, and the levels of elongating ('Ser-2' phosphorylated) RNA polymerase II in their gene bodies. Regulates muscle specificationin adult stem cells by stabilizing PAF1C in chromatin to promotemyogenic differentiation. Involved in pre-mRNA splicingas a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assemblyformed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complexupstream of the branch site is essential, it may anchor U2 snRNP to thepre-mRNA. Acts as a transcriptional regulator bybinding to the GJA1/Cx43 promoter and enhancing its up-regulation byESR1/ER-alpha.
[1-3]
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| Related KEGG Pathway | |||||||||
| Spliceosome | hsa03040 |
Pathway Map 
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| 3D Structure |
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| Function of This Protein During Virus Infection | |||||||||
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| Virus Name | SARS-COV-2 | Protein Function | Pro-viral | [4] | |||||
| Infected Tissue | Lung | Infection Time | 7-9 Days | ||||||
| Infected Cell | Calu-3 Cells (Human epithelial cell line) | Cellosaurus ID | CVCL_0609 | ||||||
| Method Description | To detect the role of host protein PHF5A in viral infection, PHF5A protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen. | ||||||||
| Results | It is reported that knockout of PHF5A leads to the decreased SARS-CoV-2 RNA levels compared with control group. | ||||||||
| Full List of Virus RNA Interacting with This Protien | |||||||||
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| RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain ) | |||||||||
| RNA Region Details |
RNA Info
Click to show the detail information of this RNA binding region
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[5] | |||||||
| Strains Name |
hCoV-19/Not Specified Virus Strain
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| RNA Binding Region |
ORF10
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| Virus Name |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
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| Infection Cells | Huh7 cells (human liver cell line) (CVCL_0336 ) | ||||||||
| Cell Originated Tissue | Liver | ||||||||
| Interaction Score | P-value < 0.05 | ||||||||
| Method Description | RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS) | ||||||||
