Host Protein General Information (ID: PT0735)
  Protein Name
PI3-kinase beta (PIK3CB)
  Gene Name
PIK3CB
  Host Species
Homo sapiens
  Uniprot Entry Name
PK3CB_HUMAN
  Protein Families
PI3/PI4-kinase family
  EC Number
2.7.1.153
  Subcellular Location
Cytoplasm Nucleus
  External Link
NCBI Gene ID
5291
Uniprot ID
P42338
Ensembl ID
ENSG00000185689
HGNC ID
HGNC:8976
  Function in Host
Phosphoinositide-3-kinase (PI3K) phosphorylatesphosphatidylinositol derivatives at position 3 of the inositol ring toproduce 3-phosphoinositides. Uses ATP andPtdIns (4, 5) P2 (phosphatidylinositol 4, 5-bisphosphate) to generatephosphatidylinositol 3, 4, 5-trisphosphate (PIP3). PIP3plays a key role by recruiting PH domain-containing proteins to themembrane, including AKT1 and PDPK1, activating signaling cascadesinvolved in cell growth, survival, proliferation, motility andmorphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine1-phosphate, and lysophosphatidic acid. May also act downstreamreceptor tyrosine kinases. Required in different signaling pathways forstable platelet adhesion and aggregation. Plays a role in plateletactivation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activationmotif) -bearing receptors such as GP6. Regulates the strength ofadhesion of ITGA2B/ ITGB3 activated receptors necessary for thecellular transmission of contractile forces. Required for plateletaggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has arole in cell survival. May have a role in cell migration. Involved inthe early stage of autophagosome formation. Modulates the intracellularlevel of PtdIns3P (phosphatidylinositol 3-phosphate) and activatesPIK3C3 kinase activity. May act as a scaffold, independently of itslipid kinase activity to positively regulate autophagy. May have a rolein insulin signaling as scaffolding protein in which the lipid kinaseactivity is not required. May have a kinase-independent function inregulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipidkinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. Has also aprotein kinase activity showing autophosphorylation. [1-5]
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  Related KEGG Pathway
Coronavirus disease - COVID-19 hsa05171            Pathway Map 
Chemokine signaling pathway hsa04062            Pathway Map 
Platelet activation hsa04611            Pathway Map 
Neutrophil extracellular trap formation hsa04613            Pathway Map 
Toll-like receptor signaling pathway hsa04620            Pathway Map 
C-type lectin receptor signaling pathway hsa04625            Pathway Map 
Natural killer cell mediated cytotoxicity hsa04650            Pathway Map 
T cell receptor signaling pathway hsa04660            Pathway Map 
B cell receptor signaling pathway hsa04662            Pathway Map 
Fc epsilon RI signaling pathway hsa04664            Pathway Map 
Fc gamma R-mediated phagocytosis hsa04666            Pathway Map 
Leukocyte transendothelial migration hsa04670            Pathway Map 
Hepatitis C hsa05160            Pathway Map 
Hepatitis B hsa05161            Pathway Map 
Measles hsa05162            Pathway Map 
Human cytomegalovirus infection hsa05163            Pathway Map 
Influenza A hsa05164            Pathway Map 
Human papillomavirus infection hsa05165            Pathway Map 
Human T-cell leukemia virus 1 infection hsa05166            Pathway Map 
Kaposi sarcoma-associated herpesvirus infection hsa05167            Pathway Map 
Herpes simplex virus 1 infection hsa05168            Pathway Map 
Epstein-Barr virus infection hsa05169            Pathway Map 
Human immunodeficiency virus 1 infection hsa05170            Pathway Map 
Viral carcinogenesis hsa05203            Pathway Map 
Bacterial invasion of epithelial cells hsa05100            Pathway Map 
Salmonella infection hsa05132            Pathway Map 
Yersinia infection hsa05135            Pathway Map 
Chagas disease hsa05142            Pathway Map 
Amoebiasis hsa05146            Pathway Map 
  3D Structure

Function of This Protein During Virus Infection
Virus NameSARS-COV-2 Protein Function Pro-viral [6]
Infected TissueLung Infection Time7-9 Days
Infected CellCalu-3 Cells (Human epithelial cell line) Cellosaurus IDCVCL_0609 
Method DescriptionTo detect the role of host protein PIK3CB in viral infection, PIK3CB protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen.
ResultsIt is reported that knockout of PIK3CB leads to the decreased SARS-CoV-2 RNA levels compared with control group.

 Full List of Virus RNA Interacting with This Protien
            RNA Region: 5'-UTR (hCoV-19/Wuhan-Hu-1/2019 )
              RNA Region Details RNA Info Click to show the detail information of this RNA binding region [7]
              Strains Name
hCoV-19/Wuhan-Hu-1/2019
              Strains Family
Beta (B.1.351)
              RNA Binding Region
5'-UTR
              Virus Name
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
              Infection Cells HEK293 Cells (Human embryonic kidney cell)  (CVCL_0045 )
              Cell Originated Tissue Liver
              Infection Time 48 h
              Interaction Score Prot score = 15
              Method Description RNA-protein interaction detection (RaPID) assay; liquid chromatography with tandem mass spectrometry (LC-MS/MS)

Differential Gene Expression During SARS-COV-2 Infection
GEO Accession: GSE152641
Sample Type: Blood
Samples Details: Healthy Control: 24; COVID-19: 62
Platform: GPL24676 Illumina NovaSeq 6000
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GEO Accession: GSE162835
Sample Type: Nasopharyngeal Swabs
Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3
Platform: GPL24676 Illumina NovaSeq 6000
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Protein Sequence Information
MCFSFIMPPAMADILDIWAVDSQIASDGSIPVDFLLPTGIYIQLEVPREATISYIKQMLWKQVHNYPMFNLLMDIDSYMFACVNQTAVYEELEDETRRLCDVRPFLPVLKLVTRSCDPGEKLDSKIGVLIGKGLHEFDSLKDPEVNEFRRKMRKFSEEKILSLVGLSWMDWLKQTYPPEHEPSIPENLEDKLYGGKLIVAVHFENCQDVFSFQVSPNMNPIKVNELAIQKRLTIHGKEDEVSPYDYVLQVSGRVEYVFGDHPLIQFQYIRNCVMNRALPHFILVECCKIKKMYEQEMIAIEAAINRNSSNLPLPLPPKKTRIISHVWENNNPFQIVLVKGNKLNTEETVKVHVRAGLFHGTELLCKTIVSSEVSGKNDHIWNEPLEFDINICDLPRMARLCFAVYAVLDKVKTKKSTKTINPSKYQTIRKAGKVHYPVAWVNTMVFDFKGQLRTGDIILHSWSSFPDELEEMLNPMGTVQTNPYTENATALHVKFPENKKQPYYYPPFDKIIEKAAEIASSDSANVSSRGGKKFLPVLKEILDRDPLSQLCENEMDLIWTLRQDCREIFPQSLPKLLLSIKWNKLEDVAQLQALLQIWPKLPPREALELLDFNYPDQYVREYAVGCLRQMSDEELSQYLLQLVQVLKYEPFLDCALSRFLLERALGNRRIGQFLFWHLRSEVHIPAVSVQFGVILEAYCRGSVGHMKVLSKQVEALNKLKTLNSLIKLNAVKLNRAKGKEAMHTCLKQSAYREALSDLQSPLNPCVILSELYVEKCKYMDSKMKPLWLVYNNKVFGEDSVGVIFKNGDDLRQDMLTLQMLRLMDLLWKEAGLDLRMLPYGCLATGDRSGLIEVVSTSETIADIQLNSSNVAAAAAFNKDALLNWLKEYNSGDDLDRAIEEFTLSCAGYCVASYVLGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFILTYDFIHVIQQGKTGNTEKFGRFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKDIQYLKDSLALGKSEEEALKQFKQKFDEALRESWTTKVNWMAHTVRKDYRS
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References
1 Cloning, expression, purification, and characterization of the human Class Ia phosphoinositide 3-kinase isoforms. Protein Expr Purif. 2004 Jun;35(2):218-24.
2 Nuclear but not cytosolic phosphoinositide 3-kinase beta has an essential function in cell survival. Mol Cell Biol. 2011 May;31(10):2122-33.
3 A biochemical mechanism for the oncogenic potential of the p110beta catalytic subunit of phosphoinositide 3-kinase. Proc Natl Acad Sci USA. 2010 Nov 16;107(46):19897-902.
4 PTEN-deficient cancers depend on PIK3CB. Proc Natl Acad Sci USA. 2008 Sep 2;105(35):13057-62.
5 Essential roles of PI(3)K-p110beta in cell growth, metabolism and tumorigenesis. Nature. 2008 Aug 7;454(7205):776-9.
6 Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2. Nat Commun. 2022 Apr 25;13(1):2237.
7 RNA-Protein Interaction Analysis of SARS-CoV-2 5 and 3 Untranslated Regions Reveals a Role of Lysosome-Associated Membrane Protein-2a during Viral Infection. mSystems. 2021 Aug 31;6(4):e0064321.