Details of Host Protein

 Host Protein General Information (ID: PT0736)
  Protein Name
Phosphatidylinositol-3-phosphatase SAC1 (IGHG1)
  Gene Name
SACM1L
  Host Species
Homo sapiens
  Uniprot Entry Name
SAC1_HUMAN
  EC Number
3.1.3.64
  Subcellular Location
Endoplasmic reticulum membrane
  External Link
NCBI Gene ID
22908
Uniprot ID
Q9NTJ5
Ensembl ID
ENSG00000211896
HGNC ID
HGNC:17059
  Function in Host
Phosphoinositide phosphatase which catalyzes the hydrolysisof phosphatidylinositol 4-phosphate (PtdIns (4) P). Can also catalyzethe hydrolysis of phosphatidylinositol 3-phosphate (PtdIns (3) P) and haslow activity towards phosphatidylinositol-3, 5-bisphosphate (PtdIns (3, 5) P2). Shows a very robust PtdIns (4) Pphosphatase activity when it binds PtdIns (4) P in a 'cis' configurationin the cellular environment, with much less activity seen when it bindsPtdIns (4) P in 'trans' configuration. PtdIns (4) P phosphatase activity (when it bindsPtdIns (4) P in 'trans' configuration) is enhanced in the presence ofPLEKHA3. [1-4]
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  Related KEGG Pathway
Phosphatidylinositol signaling system hsa04070            Pathway Map 
Inositol phosphate metabolism hsa00562            Pathway Map 
Metabolic pathways hsa01100            Pathway Map 
  3D Structure
AlphaFold ID: Q9NTJ5 
  2D PNG Download  
  mmCIF File Download   
  PDB File Download   

 Full List of Virus RNA Interacting with This Protien
            RNA Region: Not Specified Virus Region (hCoV-19/Not Specified Virus Strain )
              RNA Region Details RNA Info Click to show the detail information of this RNA binding region [5]
              Strains Name
hCoV-19/Not Specified Virus Strain
              RNA Binding Region
Not Specified Virus Region
              Virus Name
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
              Infection Cells Huh7 cells (liver carcinoma cell)  (CVCL_0336 )
              Cell Originated Tissue Liver
              Infection Time 24h
              Interaction Score log2FC = 1.49633E+14
              Method Description RNA antisense purification and quantitative mass spectrometry (RAP-MS); Tandem mass tag (TMT) labelling; liquid chromatography tandem mass spectrometry (LC-MS/MS); Westernblot

Differential Gene Expression During SARS-COV-2 Infection
GEO Accession: GSE152641
Sample Type: Blood
Samples Details: Healthy Control: 24; COVID-19: 62
Platform: GPL24676 Illumina NovaSeq 6000
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GEO Accession: GSE162835
Sample Type: Nasopharyngeal Swabs
Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3
Platform: GPL24676 Illumina NovaSeq 6000
  PNG Download  
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Protein Sequence Information
MATAAYEQLKLHITPEKFYVEACDDGADDVLTIDRVSTEVTLAVKKDVPPSAVTRPIFGILGTIHLVAGNYLIVITKKIKVGEFFSHVVWKATDFDVLSYKKTMLHLTDIQLQDNKTFLAMLNHVLNVDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYNGSKASFVQTRGSIPVFWSQRPNLKYKPLPQISKVANHMDGFQRHFDSQVIIYGKQVIINLINQKGSEKPLEQTFATMVSSLGSGMMRYIAFDFHKECKNMRWDRLSILLDQVAEMQDELSYFLVDSAGQVVANQEGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTHLGLIMDGWNSMIRYYKNNFSDGFRQDSIDLFLGNYSVDELESHSPLSVPRDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVASIGTFFIILYNGKDFVDAPRLVQKEKID
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References
1 The activity of Sac1 across ER-TGN contact sites requires the four-phosphate-adaptor-protein-1. J Cell Biol. 2019 Mar 4;218(3):783-797.
2 SAC1 degrades its lipid substrate PtdIns4 P in the endoplasmic reticulum to maintain a steep chemical gradient with donor membranes. Elife. 2018 Feb 20;7:e35588.
3 Dynamic formation of ER-PM junctions presents a lipid phosphatase to regulate phosphoinositides. J Cell Biol. 2016 Apr 11;213(1):33-48.
4 A four-step cycle driven by PI(4)P hydrolysis directs sterol/PI(4)P exchange by the ER-Golgi tether OSBP. Cell. 2013 Nov 7;155(4):830-43.
5 The SARS-CoV-2 RNA-protein interactome in infected human cells. Nat Microbiol. 2021 Mar;6(3):339-353.