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ManualDetails of Host Protein
| Host Protein General Information (ID: PT0737) | |||||||||
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| Protein Name |
Phosphatidylinositol-binding clathrin assembly protein (CALM)
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Gene Name |
PICALM
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| Host Species |
Homo sapiens
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Uniprot Entry Name |
PICAL_HUMAN
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| Protein Families |
PICALM/SNAP91 family
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| Subcellular Location |
Nucleus; plasma membrane; golgi apparatus
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| NCBI Gene ID | |||||||||
| Uniprot ID | |||||||||
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| HGNC ID | |||||||||
| Function in Host |
Cytoplasmic adapter protein that plays a critical role inclathrin-mediated endocytosis which is important in processes such asinternalization of cell receptors, synaptic transmission or removal ofapoptotic cells. Recruits AP-2 and attaches clathrin triskelions to thecytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation bydirectly sensing and driving membrane curvature. Inaddition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasmamembranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation andmaturation of autophagic precursors. Modulatesthereby autophagy and the turnover of autophagy substrates such asMAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).
[1-5]
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| 3D Structure |
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| Function of This Protein During Virus Infection | |||||||||
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| Virus Name | SARS-COV-2 | Protein Function | Pro-viral | [6] | |||||
| Infected Tissue | Lung | Infection Time | 7-9 Days | ||||||
| Infected Cell | Calu-3 Cells (Human epithelial cell line) | Cellosaurus ID | CVCL_0609 | ||||||
| Method Description | To detect the role of host protein PICALM in viral infection, PICALM protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen. | ||||||||
| Results | It is reported that knockout of PICALM leads to the decreased SARS-CoV-2 RNA levels compared with control group. | ||||||||
| Full List of Virus RNA Interacting with This Protien | |||||||||
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| RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain ) | |||||||||
| RNA Region Details |
RNA Info
Click to show the detail information of this RNA binding region
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[7] | |||||||
| Strains Name |
hCoV-19/Not Specified Virus Strain
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| RNA Binding Region |
ORF10
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| Virus Name |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
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| Infection Cells | Calu-3 cells (Human Lung Cancer Cell) (CVCL_0609 ) | ||||||||
| Cell Originated Tissue | Liver | ||||||||
| Interaction Score | P-value < 0.05 | ||||||||
| Method Description | RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS) | ||||||||
