Home
Search 
Browse 
Download
ManualDetails of Host Protein
| Host Protein General Information (ID: PT0833) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Protein Name |
Protein arginine methyltransferase 5 (PRMT5)
|
Gene Name |
PRMT5
|
||||||
| Host Species |
Homo sapiens
|
Uniprot Entry Name |
ANM5_HUMAN
|
||||||
| Protein Families |
Class I-like SAM-binding methyltransferase superfamily
|
||||||||
| EC Number |
2.1.1.32.
|
||||||||
| Subcellular Location |
Cytoplasm Nucleus Chromosome Golgi apparatus
|
||||||||
| External Link | |||||||||
| NCBI Gene ID | |||||||||
| Uniprot ID | |||||||||
| Ensembl ID | |||||||||
| HGNC ID | |||||||||
| Function in Host |
Arginine methyltransferase that can both catalyze theformation of omega-N monomethylarginine (MMA) and symmetricaldimethylarginine (sDMA), with a preference for the formation of MMA (, , , , , , , ). Specifically mediates the symmetrical dimethylation of arginineresidues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and SmD3 (SNRPD3); such methylation being required for the assembly andbiogenesis of snRNP core particles. Methylates SUPT5H and may regulate itstranscriptional elongation properties. Mono- anddimethylates arginine residues of myelin basic protein (MBP) in vitro. May play a role in cytokine-activated transduction pathways. Negativelyregulates cyclin E1 promoter activity and cellular proliferation. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may represstranscription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for theinteraction with Tudor domain-containing proteins and subsequentlocalization to the meiotic nuage. Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2levels. First, monomethylates EGFR; this enhances EGFR 'Tyr-1197'phosphorylation and PTPN6 recruitment, eventually leading to reducedSOS1 phosphorylation. Second, methylates RAF1 and probably BRAF, hence destabilizing these 2signaling proteins and reducing their catalytic activity. Required for induction of E-selectin and VCAM-1, onthe endothelial cells surface at sites of inflammation. MethylatesHOXA9. Methylates and regulates SRGAP2 which isinvolved in cell migration and differentiation. Actsas a transcriptional corepressor in CRY1-mediated repression of thecore circadian component PER1 by regulating the H4R3 dimethylation atthe PER1 promoter. Methylates GM130/GOLGA2, regulatingGolgi ribbon formation. Methylates H4R3 in genesinvolved in glioblastomagenesis in a CHTOP- and/or TET1-dependentmanner. Symmetrically methylates POLR2A, amodification that allows the recruitment to POLR2A of proteinsincluding SMN1/SMN2 and SETX. This is required for resolving RNA-DNAhybrids created by RNA polymerase II, that form R-loop in transcriptionterminal regions, an important step in proper transcription termination. Along with LYAR, binds the promoter of gamma-globinHBG1/HBG2 and represses its expression. Symmetricallymethylates NCL. Methylates p53/TP53; methylationmight possibly affect p53/TP53 target gene specificity. Involved in spliceosome maturation and mRNA splicingin prophase I spermatocytes through the catalysis of the symmetricalarginine dimethylation of SNRPB (small nuclear ribonucleoprotein-associated protein) and the interaction with tudor domain-containingprotein TDRD6.
[1-13]
Click to Show/Hide
|
||||||||
| 3D Structure |
|
||||||||
| Function of This Protein During Virus Infection | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Virus Name | SARS-COV-2 | Protein Function | Anti-viral | [14] | |||||
| Infected Tissue | Lung | Infection Time | 7-9 Days | ||||||
| Infected Cell | Calu-3 Cells (Human epithelial cell line) | Cellosaurus ID | CVCL_0609 | ||||||
| Method Description | To detect the role of host protein PRMT5 in viral infection, PRMT5 protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen. | ||||||||
| Results | It is reported that knockout of PRMT5 increases SARS-CoV-2 RNA levels compared with control group. | ||||||||
| Full List of Virus RNA Interacting with This Protien | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain ) | |||||||||
| RNA Region Details |
RNA Info
Click to show the detail information of this RNA binding region
|
[15] | |||||||
| Strains Name |
hCoV-19/Not Specified Virus Strain
|
||||||||
| RNA Binding Region |
ORF10
|
||||||||
| Virus Name |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
|
||||||||
| Infection Cells | Calu-3 cells (Human Lung Cancer Cell) (CVCL_0609 ) | ||||||||
| Cell Originated Tissue | Liver | ||||||||
| Interaction Score | P-value < 0.05 | ||||||||
| Method Description | RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS) | ||||||||
