Details of Host Protein

KEGG: hsa03008 Pathway Download ×

Host Protein General Information (ID: PT0929)
Protein Name	Ribonuclease P protein subunit p25 (RPP25)	Gene Name	RPP25
Host Species	Homo sapiens	Uniprot Entry Name	RPP25_HUMAN
Protein Families	Histone-like Alba family
Subcellular Location	Nucleus; nucleolus
External Link
	NCBI Gene ID	54913
	Uniprot ID	Q9BUL9
	Ensembl ID	ENSG00000178718
	HGNC ID	HGNC:30361
Function in Host	Component of ribonuclease P, a ribonucleoprotein complex thatgenerates mature tRNA molecules by cleaving their 5'-ends. Also a componentof the MRP ribonuclease complex, which cleaves pre-rRNA sequences. [1-4] Click to Show/Hide
Related KEGG Pathway
Related KEGG Pathway	Ribosome biogenesis in eukaryotes	hsa03008		Pathway Map
3D Structure	PDB ID: 6AHR FASTA Download 2D PNG Download PDB File Download

Function of This Protein During Virus Infection
Virus Name	SARS-COV-2	Protein Function	Pro-viral	[5]
Infected Tissue	Lung	Infection Time	7-9 Days
Infected Cell	Calu-3 Cells (Human epithelial cell line)	Cellosaurus ID	CVCL_0609
Method Description	To detect the role of host protein RPP25 in viral infection, RPP25 protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen.
Results	It is reported that knockout of RPP25 leads to the decreased SARS-CoV-2 RNA levels compared with control group.

Full List of Virus RNA Interacting with This Protien
*RNA Region: Not Specified Virus Region (hCoV-19/Not Specified Virus Strain )*
RNA Region Details	RNA Info Click to show the detail information of this RNA binding region	[6]
Strains Name	hCoV-19/Not Specified Virus Strain
RNA Binding Region	Not Specified Virus Region
Virus Name	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Infection Cells	Huh7 cells (liver carcinoma cell) (CVCL_0336 )
Cell Originated Tissue	Liver
Infection Time	24h
Interaction Score	log2FC = 485065.4438
Method Description	RNA antisense purification and quantitative mass spectrometry (RAP-MS); Tandem mass tag (TMT) labelling; liquid chromatography tandem mass spectrometry (LC-MS/MS); Westernblot

Differential Gene Expression During SARS-COV-2 Infection

GEO Accession: GSE152641

Sample Type: Blood

Samples Details: Healthy Control: 24; COVID-19: 62

Platform: GPL24676 Illumina NovaSeq 6000

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GEO Accession: GSE162835

Sample Type: Nasopharyngeal Swabs

Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3

Platform: GPL24676 Illumina NovaSeq 6000

PNG Download

GEO Accession: GSE175779

Sample Type: Human Bronchial Epithelial Cells

Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)

Platform: GPL18573 Illumina NextSeq 500

PNG Download

Protein Phosphorylation after Virus Infection
S162 [7] PNG Download

Protein Sequence Information
MENFRKVRSEEAPAGCGAEGGGPGSGPFADLAPGAVHMRVKEGSKIRNLMAFATASMAQPATRAIVFSGCGRATTKTVTCAEILKRRLAGLHQVTRLRYRSVREVWQSLPPGPTQGQTPGEPAASLSVLKNVPGLAILLSKDALDPRQPGYQPPNPHPGPSSPPAAPASKRSLGEPAAGEGSAKRSQPEPGVADEDQTA Click to Show/Hide

References
1	Cryo-EM Structure of the Human Ribonuclease P Holoenzyme. Cell. 2018 Nov 15;175(5):1393-1404.e11.
2	Targeted CRISPR disruption reveals a role for RNase MRP RNA in human preribosomal RNA processing. Genes Dev. 2017 Jan 1;31(1):59-71.
3	Differential association of protein subunits with the human RNase MRP and RNase P complexes. RNA. 2006 Jul;12(7):1373-82.
4	Purification and characterization of Rpp25, an RNA-binding protein subunit of human ribonuclease P. RNA. 2002 Mar;8(3):290-5.
5	Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2. Nat Commun. 2022 Apr 25;13(1):2237.
6	The SARS-CoV-2 RNA-protein interactome in infected human cells. Nat Microbiol. 2021 Mar;6(3):339-353.
7	Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.