Details of Host Protein

 Host Protein General Information (ID: PT0941)
  Protein Name
Ribosomal RNA-processing protein 8 (RRP8)
  Gene Name
RRP8
  Host Species
Homo sapiens
  Uniprot Entry Name
RRP8_HUMAN
  Protein Families
Methyltransferase superfamily
  EC Number
2.1.1.-
  Subcellular Location
Nucleus; nucleolus
  External Link
NCBI Gene ID
23378
Uniprot ID
O43159
Ensembl ID
ENSG00000132275
HGNC ID
HGNC:29030
  Function in Host
Essential component of the eNoSC (energy-dependent nucleolarsilencing) complex, a complex that mediates silencing of rDNA inresponse to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy statusof cell: upon glucose starvation, elevation of NAD (+) /NADP (+) ratioactivates SIRT1, leading to histone H3 deacetylation followed bydimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formationof silent chromatin in the rDNA locus. In the complex, RRP8 binds toH3K9me2 and probably acts as a methyltransferase. Its substrates arehowever unknown. [1]
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  3D Structure
PDB ID: 2ZFU 
FASTA   Download
  2D PNG Download  
PDB File Download

 Full List of Virus RNA Interacting with This Protien
            RNA Region: Not Specified Virus Region (hCoV-19/England/02/2020 )
              RNA Region Details RNA Info Click to show the detail information of this RNA binding region [2]
              Strains Name
hCoV-19/England/02/2020
              Strains Family
Beta (B.1.351)
              RNA Binding Region
Not Specified Virus Region
              Virus Name
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
              Infection Cells Calu-3 cells (Human lung cancer cell)  (CVCL_0609 )
              Cell Originated Tissue Lung
              Infection Time 24 h
              Interaction Score P-adjust = 0.059
              Method Description UV protein-RNA crosslinking; RNA interactome capture (cRIC); RNA antisense purification coupled with mass spectrometry (RAP-MS)

Differential Gene Expression During SARS-COV-2 Infection
GEO Accession: GSE152641
Sample Type: Blood
Samples Details: Healthy Control: 24; COVID-19: 62
Platform: GPL24676 Illumina NovaSeq 6000
  PNG Download  
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GEO Accession: GSE162835
Sample Type: Nasopharyngeal Swabs
Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3
Platform: GPL24676 Illumina NovaSeq 6000
  PNG Download  
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GEO Accession: GSE175779
Sample Type: Human Bronchial Epithelial Cells
Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)
Platform: GPL18573 Illumina NextSeq 500
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Protein Phosphorylation after Virus Infection
S176 [3]
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S223 [3]
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Protein Sequence Information
MFEEPEWAEAAPVAAGLGPVISRPPPAASSQNKGSKRRQLLATLRALEAASLSQHPPSLCISDSEEEEEERKKKCPKKASFASASAEVGKKGKKKCQKQGPPCSDSEEEVERKKKCHKQALVGSDSAEDEKRKRKCQKHAPINSAQHLDNVDQTGPKAWKGSTTNDPPKQSPGSTSPKPPHTLSRKQWRNRQKNKRRCKNKFQPPQVPDQAPAEAPTEKTEVSPVPRTDSHEARAGALRARMAQRLDGARFRYLNEQLYSGPSSAAQRLFQEDPEAFLLYHRGFQSQVKKWPLQPVDRIARDLRQRPASLVVADFGCGDCRLASSIRNPVHCFDLASLDPRVTVCDMAQVPLEDESVDVAVFCLSLMGTNIRDFLEEANRVLKPGGLLKVAEVSSRFEDVRTFLRAVTKLGFKIVSKDLTNSHFFLFDFQKTGPPLVGPKAQLSGLQLQPCLYKRR
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References
1 Epigenetic control of rDNA loci in response to intracellular energy status. Cell. 2008 May 16;133(4):627-39.
2 Global analysis of protein-RNA interactions in SARS-CoV-2-infected cells reveals key regulators of infection. Mol Cell. 2021 Jul 1;81(13):2851-2867.e7.
3 Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.