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ManualDetails of Host Protein
| Host Protein General Information (ID: PT0947) | |||||||||
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| Protein Name |
Nuclear export mediator factor (NEMF)
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Gene Name |
NEMF
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| Host Species |
Homo sapiens
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Uniprot Entry Name |
NEMF_HUMAN
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| Protein Families |
NEMF family
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| Subcellular Location |
Cytoplasm; cytosol Nucleus
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| External Link | |||||||||
| NCBI Gene ID | |||||||||
| Uniprot ID | |||||||||
| Ensembl ID | |||||||||
| HGNC ID | |||||||||
| Function in Host |
As part of the ribosome quality control complex (RQC), aribosome-associated complex, mediates the extraction of incompletelysynthesized nascent chains from stalled ribosomes as well as theirubiquitin-mediated proteasomal degradation. Thereby, frees ribosomesfrom the stalled translation complex and prevents the accumulation ofnascent polypeptide chains that are potentially toxic for the cell. Itis responsible both for selective recognition of stalled 60S subunitsby recognizing an exposed, nascent chain-conjugated tRNA moiety and therecruitment of the E3 ubiquitin-protein ligase LTN1 to the ribosomequality control complex. May indirectly play a rolein nuclear export.
[1-2]
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| 3D Structure |
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| Function of This Protein During Virus Infection | |||||||||
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| Virus Name | SARS-COV-2 | Protein Function | Pro-viral | [3] | |||||
| Infected Tissue | Lung | Infection Time | 7-9 Days | ||||||
| Infected Cell | Calu-3 Cells (Human epithelial cell line) | Cellosaurus ID | CVCL_0609 | ||||||
| Method Description | To detect the role of host protein NEMF in viral infection, NEMF protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen. | ||||||||
| Results | It is reported that knockout of NEMF leads to the decreased SARS-CoV-2 RNA levels compared with control group. | ||||||||
| Full List of Virus RNA Interacting with This Protien | |||||||||
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| RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain ) | |||||||||
| RNA Region Details |
RNA Info
Click to show the detail information of this RNA binding region
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[4] | |||||||
| Strains Name |
hCoV-19/Not Specified Virus Strain
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| RNA Binding Region |
ORF10
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| Virus Name |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
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| Infection Cells | Calu-3 cells (Human Lung Cancer Cell) (CVCL_0609 ) | ||||||||
| Cell Originated Tissue | Liver | ||||||||
| Interaction Score | P-value < 0.05 | ||||||||
| Method Description | RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS) | ||||||||
