Details of Host Protein

 Host Protein General Information (ID: PT1033)
  Protein Name
Serine/threonine-protein kinase B-raf (BRAF)
  Gene Name
BRAF
  Host Species
Homo sapiens
  Uniprot Entry Name
BRAF_HUMAN
  Protein Families
Protein kinase superfamily
  EC Number
2.7.11.1
  Subcellular Location
Nucleus Cytoplasm Cell membrane
  External Link
NCBI Gene ID
673
Uniprot ID
P15056
Ensembl ID
ENSG00000157764
HGNC ID
HGNC:1097
  Function in Host
Protein kinase involved in the transduction of mitogenicsignals from the cell membrane to the nucleus. Phosphorylates MAP2K1, and thereby activates the MAP kinase signaltransduction pathway. May play arole in the postsynaptic responses of hippocampal neurons. [1-3]
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  Related KEGG Pathway
Chemokine signaling pathway hsa04062            Pathway Map 
Natural killer cell mediated cytotoxicity hsa04650            Pathway Map 
Hepatitis C hsa05160            Pathway Map 
Hepatitis B hsa05161            Pathway Map 
Alcoholism hsa05034            Pathway Map 
Non-small cell lung cancer hsa05223            Pathway Map 
MAPK signaling pathway hsa04010            Pathway Map 
ErbB signaling pathway hsa04012            Pathway Map 
Rap1 signaling pathway hsa04015            Pathway Map 
  3D Structure
PDB ID: 1UWH 
FASTA   Download
  2D PNG Download  
PDB File Download

 Full List of Virus RNA Interacting with This Protien
            RNA Region: 5'-UTR (hCoV-19/Wuhan-Hu-1/2019 )
              RNA Region Details RNA Info Click to show the detail information of this RNA binding region [4]
              Strains Name
hCoV-19/Wuhan-Hu-1/2019
              Strains Family
Beta (B.1.351)
              RNA Binding Region
5'-UTR
              Virus Name
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
              Infection Cells HEK293 Cells (Human embryonic kidney cell)  (CVCL_0045 )
              Cell Originated Tissue Liver
              Infection Time 48 h
              Interaction Score Prot score = 16
              Method Description RNA-protein interaction detection (RaPID) assay; liquid chromatography with tandem mass spectrometry (LC-MS/MS)

Differential Gene Expression During SARS-COV-2 Infection
GEO Accession: GSE152641
Sample Type: Blood
Samples Details: Healthy Control: 24; COVID-19: 62
Platform: GPL24676 Illumina NovaSeq 6000
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GEO Accession: GSE162835
Sample Type: Nasopharyngeal Swabs
Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3
Platform: GPL24676 Illumina NovaSeq 6000
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GEO Accession: GSE175779
Sample Type: Human Bronchial Epithelial Cells
Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)
Platform: GPL18573 Illumina NextSeq 500
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Protein Phosphorylation after Virus Infection
S151 [5]
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S151 [6]
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S365 [6]
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S399 [6]
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S419 [5]
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S446 [5]
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S729 [6]
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T395 [6]
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T401 [6]
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Potential Drug(s) that Targets This Protein
Drug Name DrunkBank ID Pubchem ID TTD ID REF
Dabrafenib DB08912  44462760  D05ROI  [5]
Fostamatinib DB12010  11671467  D0V8HJ  [7]
Regorafenib DB08896  11167602  D09GDD  [6]
Sorafenib DB00398  406563  . [5]
Vemurafenib DB08881  42611257  D0Y9EW  [6]

Protein Sequence Information
MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH
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References
1 MEK drives BRAF activation through allosteric control of KSR proteins. Nature. 2018 Feb 22;554(7693):549-553.
2 A Raf-induced allosteric transition of KSR stimulates phosphorylation of MEK. Nature. 2011 Apr 21;472(7343):366-9.
3 95-kilodalton B-Raf serine/threonine kinase: identification of the protein and its major autophosphorylation site. Mol Cell Biol. 1992 Sep;12(9):3733-42.
4 RNA-Protein Interaction Analysis of SARS-CoV-2 5 and 3 Untranslated Regions Reveals a Role of Lysosome-Associated Membrane Protein-2a during Viral Infection. mSystems. 2021 Aug 31;6(4):e0064321.
5 The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell. 2020 Aug 6;182(3):685-712.e19.
6 Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.
7 DrugBank