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ManualDetails of Host Protein
| Host Protein General Information (ID: PT1048) | |||||||||
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| Protein Name |
Interferon-regulated antiviral protein (IRAV)
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Gene Name |
SHFL
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| Host Species |
Homo sapiens
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Uniprot Entry Name |
SHFL_HUMAN
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| Protein Families |
SHFL family
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| Subcellular Location |
Cytoplasm Nucleus Cytoplasm; P-body
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| External Link | |||||||||
| NCBI Gene ID | |||||||||
| Uniprot ID | |||||||||
| Ensembl ID | |||||||||
| HGNC ID | |||||||||
| Function in Host |
Inhibits programmed -1 ribosomal frameshifting (-1PRF) of avariety of mRNAs from viruses, such as HIV1, and cellular genes, suchas PEG10. Interacts with the -1PRF signal of target mRNA andtranslating ribosomes and causes premature translation termination atthe frameshifting site. Regulates HIV1 GAG-POLexpression by inhibiting -1PRF. Exhibits antiviralactivity against dengue virus (DENV) and can inhibit the replication ofall DENV serotypes. May block the protein translation of DENV RNA viaits association with cellular mRNA-binding proteins and viral RNA. Interrupts also Zika virus replication by promoting viral NS3degradation via a lysosome-dependent pathway. Canalso limit the replication of hepatitis C virus (HCV) by restrictingformation of viral replication organelle, West Nile virus (WNV), Chikungunya virus (CHIKV), herpes simplex virus type 1 (HHV-1), herpesvirus type 8 (HHV-8) and human adenovirus. Binds nucleic acidswith a higher affinity for ssRNA and ssDNA than for dsDNA.
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| 3D Structure |
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| Function of This Protein During Virus Infection | |||||||||
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| Virus Name | SARS-COV-2 | Protein Function | Pro-viral | [1] | |||||
| Infected Tissue | Lung | Infection Time | 7-9 Days | ||||||
| Infected Cell | Calu-3 Cells (Human epithelial cell line) | Cellosaurus ID | CVCL_0609 | ||||||
| Method Description | To detect the role of host protein SHFL in viral infection, SHFL protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen. | ||||||||
| Results | It is reported that knockout of SHFL leads to the decreased SARS-CoV-2 RNA levels compared with control group. | ||||||||
| Full List of Virus RNA Interacting with This Protien | |||||||||
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| RNA Region: Not Specified Virus Region (hCov-OC43/VR-759 Quebec/2019 ) | |||||||||
| RNA Region Details |
RNA Info
Click to show the detail information of this RNA binding region
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[2] | |||||||
| Strains Name |
hCov-OC43/VR-759 Quebec/2019
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| Strains Family |
Beta
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| RNA Binding Region |
Not Specified Virus Region
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| Virus Name |
Human coronavirus OC43 (HCov-OC43)
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| Infection Cells | Vero cells (epithelial kidney cell) (CVCL_0059 ) | ||||||||
| Cell Originated Tissue | kidney | ||||||||
| Infection Time | 24 h | ||||||||
| Interaction Score | adj. p_value < 0.05 | ||||||||
| Method Description | Modified RNA antisense purification coupled with mass spectrometry (RAP-MS); label-free quantification (LFQ); liquid chromatography with tandem mass spectrometry (LC-MS/MS) | ||||||||
