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ManualDetails of Host Protein
| Host Protein General Information (ID: PT1088) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Protein Name |
Splicing factor 3B subunit 6 (SF3B6)
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Gene Name |
SF3B6
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| Host Species |
Homo sapiens
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Uniprot Entry Name |
SF3B6_HUMAN
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| Protein Families |
SF3B6 family
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| Subcellular Location |
Nucleus
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| External Link | |||||||||
| NCBI Gene ID | |||||||||
| Uniprot ID | |||||||||
| Ensembl ID | |||||||||
| HGNC ID | |||||||||
| Function in Host |
Involved in pre-mRNA splicing as a component of the splicingfactor SF3B complex. SF3B complex is required for 'A'complex assembly formed by the stable binding of U2 snRNP to thebranchpoint sequence (BPS) in pre-mRNA. Directlycontacts the pre-mRNA branch site adenosine for the first catalyticstep of splicing. Enters the spliceosome andassociates with the pre-mRNA branch site as part of the 17S U2 or, inthe case of the minor spliceosome, as part of the 18S U11/U12 snRNPcomplex, and thus may facilitate the interaction of these snRNP withthe branch sites of U2 and U12 respectively.
[1-3]
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| Related KEGG Pathway | |||||||||
| Spliceosome | hsa03040 |
Pathway Map 
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| 3D Structure |
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| Function of This Protein During Virus Infection | |||||||||
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| Virus Name | SARS-COV-2 | Protein Function | Anti-viral | [4] | |||||
| Infected Tissue | Lung | Infection Time | 7-9 Days | ||||||
| Infected Cell | Calu-3 Cells (Human epithelial cell line) | Cellosaurus ID | CVCL_0609 | ||||||
| Method Description | To detect the role of host protein SF3B6 in viral infection, SF3B6 protein knockdown Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen. | ||||||||
| Results | It is reported that knockout of SF3B6 increases SARS-CoV-2 RNA levels compared with control group. | ||||||||
| Full List of Virus RNA Interacting with This Protien | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain ) | |||||||||
| RNA Region Details |
RNA Info
Click to show the detail information of this RNA binding region
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[5] | |||||||
| Strains Name |
hCoV-19/Not Specified Virus Strain
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| RNA Binding Region |
ORF10
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| Virus Name |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
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| Infection Cells | Huh7 cells (human liver cell line) (CVCL_0336 ) | ||||||||
| Cell Originated Tissue | Liver | ||||||||
| Interaction Score | P-value < 0.05 | ||||||||
| Method Description | RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS) | ||||||||
