Details of Host Protein

KEGG: hsa00010 Pathway Download ×

KEGG: hsa00051 Pathway Download ×

KEGG: hsa00562 Pathway Download ×

KEGG: hsa01100 Pathway Download ×

KEGG: hsa01200 Pathway Download ×

KEGG: hsa01230 Pathway Download ×

Host Protein General Information (ID: PT1176)
Protein Name	Triosephosphate isomerase (TPI1)	Gene Name	TPI1
Host Species	Homo sapiens	Uniprot Entry Name	TPIS_HUMAN
Protein Families	Triosephosphate isomerase family
EC Number	5.3.1.1; 4.2.3.3
Subcellular Location	Cytoplasm
External Link
	NCBI Gene ID	7167
	Uniprot ID	P60174
	Ensembl ID	ENSG00000111669
	HGNC ID	HGNC:12009
Function in Host	Triosephosphate isomerase is an extremely efficient metabolicenzyme that catalyzes the interconversion between dihydroxyacetonephosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysisand gluconeogenesis. [1] Click to Show/Hide
Related KEGG Pathway
	Glycolysis / Gluconeogenesis	hsa00010		Pathway Map
	Fructose and mannose metabolism	hsa00051		Pathway Map
	Inositol phosphate metabolism	hsa00562		Pathway Map
	Metabolic pathways	hsa01100		Pathway Map
	Carbon metabolism	hsa01200		Pathway Map
	Biosynthesis of amino acids	hsa01230		Pathway Map
3D Structure	PDB ID: 1HTI FASTA Download 2D PNG Download PDB File Download

Function of This Protein During Virus Infection
Virus Name	SARS-COV-2	Protein Function	Anti-viral	[2]
Infected Tissue	Lung	Infection Time	7-9 Days
Infected Cell	Calu-3 Cells (Human epithelial cell line)	Cellosaurus ID	CVCL_0609
Method Description	To detect the role of host protein TPI1 in viral infection, TPI1 protein Knockdown Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen.
Results	It is reported that knockout of TPI1 increases SARS-CoV-2 RNA levels compared with control group.

Full List of Virus RNA Interacting with This Protien
*RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain )*
RNA Region Details	RNA Info Click to show the detail information of this RNA binding region	[3]
Strains Name	hCoV-19/Not Specified Virus Strain
RNA Binding Region	ORF10
Virus Name	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Infection Cells	Huh7 cells (human liver cell line) (CVCL_0336 )
Cell Originated Tissue	Liver
Interaction Score	P-value < 0.05
Method Description	RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS)

Differential Gene Expression During SARS-COV-2 Infection

GEO Accession: GSE152641

Sample Type: Blood

Samples Details: Healthy Control: 24; COVID-19: 62

Platform: GPL24676 Illumina NovaSeq 6000

PNG Download

GEO Accession: GSE162835

Sample Type: Nasopharyngeal Swabs

Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3

Platform: GPL24676 Illumina NovaSeq 6000

PNG Download

GEO Accession: GSE175779

Sample Type: Human Bronchial Epithelial Cells

Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)

Platform: GPL18573 Illumina NextSeq 500

PNG Download

Protein Phosphorylation after Virus Infection
S117 [4] PNG Download
S249 [4] PNG Download
S58 [4] PNG Download
T251 [4] PNG Download

Protein Sequence Information
MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ Click to Show/Hide

References
1	Structural basis of human triosephosphate isomerase deficiency: mutation E104D is related to alterations of a conserved water network at the dimer interface. J Biol Chem. 2008 Aug 22;283(34):23254-63.
2	Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2. Nat Commun. 2022 Apr 25;13(1):2237.
3	Mapping the host protein interactome of non-coding regions in SARS-CoV-2 genome. bioRxiv. 2021 Jun; DOI:10.1101/2021.06.19.449092.
4	Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.