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ManualDetails of Host Protein
| Host Protein General Information (ID: PT1180) | |||||||||
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| Protein Name |
tRNA methyltransferase 10 homolog C (TRMT10C)
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Gene Name |
TRMT10C
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| Host Species |
Homo sapiens
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Uniprot Entry Name |
TM10C_HUMAN
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| Protein Families |
Class IV-like SAM-binding methyltransferase superfamily
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| EC Number |
2.1.1.-; 2.1.1.218; 2.1.1.221
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| Subcellular Location |
Mitochondrion matrix; mitochondrion nucleoid
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| External Link | |||||||||
| NCBI Gene ID | |||||||||
| Uniprot ID | |||||||||
| Ensembl ID | |||||||||
| HGNC ID | |||||||||
| Function in Host |
Mitochondrial tRNA N (1) -methyltransferase involved inmitochondrial tRNA maturation. Component of mitochondrialribonuclease P, a complex composed of TRMT10C/MRPP1, HSD17B10/MRPP2 andPRORP/MRPP3, which cleaves tRNA molecules in their 5'-ends. Together with HSD17B10/MRPP2, forms a subcomplex ofthe mitochondrial ribonuclease P, named MRPP1-MRPP2 subcomplex, whichdisplays functions that are independent of the ribonuclease P activity. The MRPP1-MRPP2 subcomplexcatalyzes the formation of N (1) -methylguanine and N (1) -methyladenine atposition 9 (m1G9 and m1A9, respectively) in tRNAs; TRMT10C/MRPP1 actingas the catalytic N (1) -methyltransferase subunit. TheMRPP1-MRPP2 subcomplex also acts as a tRNA maturation platform:following 5'-end cleavage by the mitochondrial ribonuclease P complex, the MRPP1-MRPP2 subcomplex enhances the efficiency of 3'-processingcatalyzed by ELAC2, retains the tRNA product after ELAC2 processing andpresents the nascent tRNA to the mitochondrial CCA tRNAnucleotidyltransferase TRNT1 enzyme. In addition totRNA N (1) -methyltransferase activity, TRMT10C/MRPP1 also acts as a mRNAN (1) -methyltransferase by mediating methylation of adenosine residuesat the N (1) position of MT-ND5 mRNA. Associates withmitochondrial DNA complexes at the nucleoids to initiate RNA processingand ribosome assembly.
[1-5]
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| 3D Structure |
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| Function of This Protein During Virus Infection | |||||||||
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| Virus Name | SARS-COV-2 | Protein Function | Anti-viral | [6] | |||||
| Infected Tissue | Lung | Infection Time | 7-9 Days | ||||||
| Infected Cell | Calu-3 Cells (Human epithelial cell line) | Cellosaurus ID | CVCL_0609 | ||||||
| Method Description | To detect the role of host protein TRMT10C in viral infection, TRMT10C protein knockdown Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen. | ||||||||
| Results | It is reported that knockout of TRMT10C increases SARS-CoV-2 RNA levels compared with control group. | ||||||||
| Full List of Virus RNA Interacting with This Protien | |||||||||
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| RNA Region: Not Specified Virus Region (hCoV-19/England/02/2020 ) | |||||||||
| RNA Region Details |
RNA Info
Click to show the detail information of this RNA binding region
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[7] | |||||||
| Strains Name |
hCoV-19/England/02/2020
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| Strains Family |
Beta (B.1.351)
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| RNA Binding Region |
Not Specified Virus Region
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| Virus Name |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
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| Infection Cells | Calu-3 cells (Human lung cancer cell) (CVCL_0609 ) | ||||||||
| Cell Originated Tissue | Lung | ||||||||
| Infection Time | 24 h | ||||||||
| Interaction Score | P-adjust = 0.030 | ||||||||
| Method Description | UV protein-RNA crosslinking; RNA interactome capture (cRIC); RNA antisense purification coupled with mass spectrometry (RAP-MS) | ||||||||
