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ManualDetails of Host Protein
| Host Protein General Information (ID: PT1194) | |||||||||
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| Protein Name |
Tudor-interacting repair regulator protein (NUDT16L1)
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Gene Name |
NUDT16L1
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| Host Species |
Homo sapiens
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Uniprot Entry Name |
TIRR_HUMAN
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| Protein Families |
Nudix hydrolase family
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| Subcellular Location |
Nucleus
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| External Link | |||||||||
| NCBI Gene ID | |||||||||
| Uniprot ID | |||||||||
| Ensembl ID | |||||||||
| HGNC ID | |||||||||
| Function in Host |
Key regulator of TP53BP1 required to stabilize TP53BP1 andregulate its recruitment to chromatin. In absence ofDNA damage, interacts with the tandem Tudor-like domain of TP53BP1, masking the region that binds histone H4 dimethylated at 'Lys-20' (H4K20me2), thereby preventing TP53BP1 recruitment to chromatin andmaintaining TP53BP1 localization to the nucleus. Following DNA damage, ATM-induced phosphorylation of TP53BP1 andsubsequent recruitment of RIF1 leads to dissociate NUDT16L1/TIRR fromTP53BP1, unmasking the tandem Tudor-like domain and allowingrecruitment of TP53BP1 to DNA double strand breaks (DSBs). Binds U8 snoRNA.
[1-2]
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| Related KEGG Pathway | |||||||||
| Proteoglycans in cancer | hsa05205 |
Pathway Map 
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| Proteoglycans in cancer | hsa05205 |
Pathway Map
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| 3D Structure |
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| Function of This Protein During Virus Infection | |||||||||
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| Virus Name | SARS-COV-2 | Protein Function | Anti-viral | [3] | |||||
| Infected Tissue | Lung | Infection Time | 7-9 Days | ||||||
| Infected Cell | Calu-3 Cells (Human epithelial cell line) | Cellosaurus ID | CVCL_0609 | ||||||
| Method Description | To detect the role of host protein NUDT16L1 in viral infection, NUDT16L1 protein Knockdown Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen. | ||||||||
| Results | It is reported that knockout of NUDT16L1 increases SARS-CoV-2 RNA levels compared with control group. | ||||||||
| Full List of Virus RNA Interacting with This Protien | |||||||||
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| RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain ) | |||||||||
| RNA Region Details |
RNA Info
Click to show the detail information of this RNA binding region
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[4] | |||||||
| Strains Name |
hCoV-19/Not Specified Virus Strain
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| RNA Binding Region |
ORF10
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| Virus Name |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
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| Infection Cells | Huh7 cells (human liver cell line) (CVCL_0336 ) | ||||||||
| Cell Originated Tissue | Liver | ||||||||
| Interaction Score | P-value < 0.05 | ||||||||
| Method Description | RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS) | ||||||||
