Details of Host Protein

 Host Protein General Information (ID: PT1240)
  Protein Name
Trans-2,3-enoyl-CoA reductase (TER)
  Gene Name
TECR
  Host Species
Homo sapiens
  Uniprot Entry Name
TECR_HUMAN
  Protein Families
Steroid 5-alpha reductase family
  EC Number
1.3.1.93
  Subcellular Location
Endoplasmic reticulum membrane
  External Link
NCBI Gene ID
9524
Uniprot ID
Q9NZ01
Ensembl ID
ENSG00000099797
HGNC ID
HGNC:4551
  Function in Host
Involved in both the production of very long-chain fattyacids for sphingolipid synthesis and the degradation of the sphingosinemoiety in sphingolipids through the sphingosine 1-phosphate metabolicpathway. Catalyzes the last of the four reactions ofthe long-chain fatty acids elongation cycle. Thisendoplasmic reticulum-bound enzymatic process, allows the addition of 2carbons to the chain of long- and very long-chain fatty acids/VLCFAsper cycle. This enzyme reduces the trans-2, 3-enoyl-CoA fatty acid intermediate to an acyl-CoA that can be furtherelongated by entering a new cycle of elongation. Thereby, it participates in the production of VLCFAs of different chainlengths that are involved in multiple biological processes asprecursors of membrane lipids and lipid mediators. Catalyzes the saturation step of the sphingosine 1-phosphate metabolicpathway, the conversion of trans-2-hexadecenoyl-CoA to palmitoyl-CoA. [1-2]
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  Related KEGG Pathway
Metabolic pathways hsa01100            Pathway Map 
Fatty acid metabolism hsa01212            Pathway Map 
Fatty acid elongation hsa00062            Pathway Map 
Biosynthesis of unsaturated fatty acids hsa01040            Pathway Map 
Metabolic pathways hsa01100            Pathway Map 
Fatty acid metabolism hsa01212            Pathway Map 
Fatty acid elongation hsa00062            Pathway Map 
Biosynthesis of unsaturated fatty acids hsa01040            Pathway Map 
  3D Structure
PDB ID: 2DZJ 
FASTA   Download
  2D PNG Download  
PDB File Download

Function of This Protein During Virus Infection
Virus NameSARS-COV-2 Protein Function Pro-viral [3]
Infected TissueLung Infection Time7-9 Days
Infected CellCalu-3 Cells (Human epithelial cell line) Cellosaurus IDCVCL_0609 
Method DescriptionTo detect the role of host protein TECR in viral infection, TECR protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen.
ResultsIt is reported that knockout of TECR leads to the decreased SARS-CoV-2 RNA levels compared with control group.

 Full List of Virus RNA Interacting with This Protien
            RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain )
              RNA Region Details RNA Info Click to show the detail information of this RNA binding region [4]
              Strains Name
hCoV-19/Not Specified Virus Strain
              RNA Binding Region
ORF10
              Virus Name
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
              Infection Cells Huh7 cells (human liver cell line)  (CVCL_0336 )
              Cell Originated Tissue Liver
              Interaction Score P-value < 0.05
              Method Description RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS)

Differential Gene Expression During SARS-COV-2 Infection
GEO Accession: GSE152641
Sample Type: Blood
Samples Details: Healthy Control: 24; COVID-19: 62
Platform: GPL24676 Illumina NovaSeq 6000
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GEO Accession: GSE162835
Sample Type: Nasopharyngeal Swabs
Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3
Platform: GPL24676 Illumina NovaSeq 6000
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GEO Accession: GSE175779
Sample Type: Human Bronchial Epithelial Cells
Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)
Platform: GPL18573 Illumina NextSeq 500
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Protein Sequence Information
MKHYEVEILDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPVGTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGHKYDFTSSRHTVVHLACICHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKHRSYLKEFRDYPPLRMPIIPFLL
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References
1 Dual functions of the trans-2-enoyl-CoA reductase TER in the sphingosine 1-phosphate metabolic pathway and in fatty acid elongation. J Biol Chem. 2014 Sep 5;289(36):24736-48.
2 Identification of two mammalian reductases involved in the two-carbon fatty acyl elongation cascade. J Biol Chem. 2003 Feb 28;278(9):7335-43.
3 Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2. Nat Commun. 2022 Apr 25;13(1):2237.
4 Mapping the host protein interactome of non-coding regions in SARS-CoV-2 genome. bioRxiv. 2021 Jun; DOI:10.1101/2021.06.19.449092.