Details of Host Protein

KEGG: hsa05171 Pathway Download ×

KEGG: hsa03010 Pathway Download ×

Host Protein General Information (ID: PT0070)
Protein Name		40S ribosomal protein S17 (RPS16)			Gene Name		RPS16
Host Species		Homo sapiens			Uniprot Entry Name		RS16_HUMAN
Protein Families		Universal ribosomal protein uS9 family
External Link
		NCBI Gene ID			6217
		Uniprot ID			P62249
		Ensembl ID			ENSG00000105193
		HGNC ID			HGNC:10396
Related KEGG Pathway
		Coronavirus disease - COVID-19			hsa05171			Pathway Map
		Ribosome			hsa03010			Pathway Map
3D Structure		PDB ID: 4UG0  FASTA   Download   2D PNG Download   PDB File Download

Function of This Protein During Virus Infection
Virus Name		SARS-COV-2			Protein Function		Anti-viral		[1]
Infected Tissue		Lung			Infection Time		7-9 Days
Infected Cell		Calu-3 Cells (Human epithelial cell line)			Cellosaurus ID		CVCL_0609
Method Description		To detect the role of host protein RPS16 in viral infection, RPS16 protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen.
Results		It is reported that knockout of RPS16 increases SARS-CoV-2 RNA levels compared with control group.

Host Protein - Virus RNA Network
Network Download

Full List of Virus RNA Interacting with This Protien
RNA Region: 3'-UTR (hCoV-19/IPBCAMS-YL01/2020 )
RNA Region Details			RNA Info Click to show the detail information of this RNA binding region						[2]
Strains Name			hCoV-19/IPBCAMS-YL01/2020
Strains Family			Beta (B.1.351)
RNA Binding Region			3'-UTR
Virus Name			Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Interaction Type			Potiential to be direct binder
Infection Cells			Huh7.5.1 cells (Hepatocyte derived cellular carcinoma cell)  (CVCL_E049 )
Cell Originated Tissue			Liver
Infection Time			30 h
Interaction Score			MIST = 0.68552103
Method Description			comprehensive identification of RNA-binding proteins by massspectrometry (ChIRP-MS)
RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain )
RNA Region Details			RNA Info Click to show the detail information of this RNA binding region						[3]
Strains Name			hCoV-19/Not Specified Virus Strain
RNA Binding Region			ORF10
Virus Name			Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Infection Cells			Huh7 cells (human liver cell line) Huh7 cells (human liver cell line)  (CVCL_0336 )
Cell Originated Tissue			Liver
Interaction Score			P-value < 0.05
Method Description			RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS)
RNA Region: Not Specified Virus Region (hCoV-19/France/IDF-220-95/2020 )
RNA Region Details			RNA Info Click to show the detail information of this RNA binding region						[4]
Strains Name			hCoV-19/France/IDF-220-95/2020
RNA Binding Region			Not Specified Virus Region
Virus Name			Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Interaction Type			Direct interaction
Infection Cells			HEK293 Cells (Human embryonic kidney cell) HEK293 Cells (Human embryonic kidney cell)  (CVCL_0045 )
Cell Originated Tissue			Kidney
Infection Time			48 h
Interaction Score			SAINT score ≥ 0.79
Method Description			comprehensive identification of RNA-binding proteins by massspectrometry (ChIRP-MS)
RNA Region: Not Specified Virus Region (hCoV-19/England/02/2020 )
RNA Region Details			RNA Info Click to show the detail information of this RNA binding region						[5]
Strains Name			hCoV-19/England/02/2020
Strains Family			Beta (B.1.351)
RNA Binding Region			Not Specified Virus Region
Virus Name			Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Infection Cells			Calu-3 cells (Human lung cancer cell) Calu-3 cells (Human lung cancer cell)  (CVCL_0609 )
Cell Originated Tissue			Lung
Infection Time			24 h
Interaction Score			P-adjust = 0.051
Method Description			UV protein-RNA crosslinking; RNA interactome capture (cRIC); RNA antisense purification coupled with mass spectrometry (RAP-MS)

Differential Gene Expression During SARS-COV-2 Infection

GEO Accession: GSE152641 Sample Type: Blood Samples Details: Healthy Control: 24; COVID-19: 62 Platform: GPL24676 Illumina NovaSeq 6000   PNG Download   GEO Accession: GSE162835 Sample Type: Nasopharyngeal Swabs Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3 Platform: GPL24676 Illumina NovaSeq 6000   PNG Download   GEO Accession: GSE175779 Sample Type: Human Bronchial Epithelial Cells Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h) Platform: GPL18573 Illumina NextSeq 500   PNG Download

Protein Phosphorylation after Virus Infection
S3 [6]   PNG Download

Protein Sequence Information
MPSKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR     Click to Show/Hide

References
1	Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2. Nat Commun. 2022 Apr 25;13(1):2237.
2	Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. Cell Res. 2022 Jan;32(1):9-23.
3	Mapping the host protein interactome of non-coding regions in SARS-CoV-2 genome. bioRxiv. 2021 Jun; DOI:10.1101/2021.06.19.449092.
4	Characterization and functional interrogation of the SARS-CoV-2 RNA interactome. Cell Rep. 2022 Apr 26;39(4):110744.
5	Global analysis of protein-RNA interactions in SARS-CoV-2-infected cells reveals key regulators of infection. Mol Cell. 2021 Jul 1;81(13):2851-2867.e7.
6	The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell. 2020 Aug 6;182(3):685-712.e19.