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ManualDetails of Host Protein
| Host Protein General Information (ID: PT0123) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Protein Name |
60S ribosomal protein L6 (RPL5)
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Gene Name |
RPL5
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| Host Species |
Homo sapiens
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Uniprot Entry Name |
RL5_HUMAN
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| Protein Families |
Universal ribosomal protein uL18 family
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| Subcellular Location |
Cytoplasm
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| External Link | |||||||||
| NCBI Gene ID | |||||||||
| Uniprot ID | |||||||||
| Ensembl ID | |||||||||
| HGNC ID | |||||||||
| Function in Host |
Component of the ribosome, a large ribonucleoprotein complexresponsible for the synthesis of proteins in the cell. The smallribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates theencoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalyticsite termed the peptidyl transferase center (PTC), which catalyzes theformation of peptide bonds, thereby polymerizing the amino acidsdelivered by tRNAs into a polypeptide chain. The nascent polypeptidesleave the ribosome through a tunnel in the LSU and interact withprotein factors that function in enzymatic processing, targeting, andthe membrane insertion of nascent chains at the exit of the ribosomaltunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is anessential component of the LSU, required for its formation and thematuration of rRNAs. It also couples ribosome biogenesis to p53/TP53activation. As part of the 5S RNP it accumulates in the nucleoplasm andinhibits MDM2, when ribosome biogenesis is perturbed, mediating thestabilization and the activation of TP53.
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| Related KEGG Pathway | |||||||||
| Coronavirus disease - COVID-19 | hsa05171 |
Pathway Map 
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| Ribosome | hsa03010 |
Pathway Map 
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| 3D Structure |
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| Function of This Protein During Virus Infection | |||||||||
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| Virus Name | SARS-COV-2 | Protein Function | Anti-viral | [5] | |||||
| Infected Tissue | Lung | Infection Time | 7-9 Days | ||||||
| Infected Cell | Calu-3 Cells (Human epithelial cell line) | Cellosaurus ID | CVCL_0609 | ||||||
| Method Description | To detect the role of host protein RPL5 in viral infection, RPL5 protein knockout Calu-3 Cells were infected with SARS-COV-2 for 7 - 9 Days , and the effects on infection was detected through CRISPR-based genome-wide gene-knockout screen. | ||||||||
| Results | It is reported that knockout of RPL5 increases SARS-CoV-2 RNA levels compared with control group. | ||||||||
| Host Protein - Virus RNA Network | |||||||||
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| Full List of Virus RNA Interacting with This Protien | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| RNA Region: 5'-UTR (hCoV-19/South Korea/KCDC03/2020 ) | |||||||||
| RNA Region Details |
RNA Info
Click to show the detail information of this RNA binding region
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[6] | |||||||
| Strains Name |
hCoV-19/South Korea/KCDC03/2020
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| Strains Family |
Alpha (B.1.1.7)
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| RNA Binding Region |
5'-UTR
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| Virus Name |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
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| Interaction Type | Directly bind to SARS-CoV-23 RNA's 5' UTR region | ||||||||
| Infection Cells | Vero cells (epithelial kidney cell) (CVCL_0059 ) | ||||||||
| Cell Originated Tissue | kidney | ||||||||
| Infection Time | 24 h | ||||||||
| Interaction Score | P-adjust < 0.05 | ||||||||
| Method Description | Modified RNA antisense purification coupled with mass spectrometry (RAP-MS); label-free quantification (LFQ); liquid chromatography with tandem mass spectrometry (LC-MS/MS) | ||||||||
| RNA Region: ORF10 (hCoV-19/Not Specified Virus Strain ) | |||||||||
| RNA Region Details |
RNA Info
Click to show the detail information of this RNA binding region
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[7] | |||||||
| Strains Name |
hCoV-19/Not Specified Virus Strain
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| RNA Binding Region |
ORF10
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| Virus Name |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
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| Infection Cells | Huh7 cells (human liver cell line) Huh7 cells (human liver cell line) (CVCL_0336 ) | ||||||||
| Cell Originated Tissue | Liver | ||||||||
| Interaction Score | P-value < 0.05 | ||||||||
| Method Description | RNA pull-down assays; liquid chromatography with tandem mass spectrometry (LC-MS/MS); Wilcoxon test; MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS) | ||||||||
| RNA Region: Not Specified Virus Region (hCov-OC43/VR-759 Quebec/2019 ) | |||||||||
| RNA Region Details |
RNA Info
Click to show the detail information of this RNA binding region
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[6] | |||||||
| Strains Name |
hCov-OC43/VR-759 Quebec/2019
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| Strains Family |
Beta
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| RNA Binding Region |
Not Specified Virus Region
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| Virus Name |
Human coronavirus OC43 (HCov-OC43)
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| Infection Cells | HCT-8 cells (Human ileocaecal adenocarcinoma cell) HCT-8 cells (Human ileocaecal adenocarcinoma cell) (CVCL_2478 ) | ||||||||
| Cell Originated Tissue | Ileocecum | ||||||||
| Infection Time | 12h; 24; 36h; 48h | ||||||||
| Interaction Score | p_value < 0.05; FDR < 10% | ||||||||
| Method Description | Modified RNA antisense purification coupled with mass spectrometry (RAP-MS); label-free quantification (LFQ); liquid chromatography with tandem mass spectrometry (LC-MS/MS) | ||||||||
