Details of Host Protein

 Host Protein General Information (ID: PT0914)
  Protein Name
Ras-related protein Rab-7a (RAB7A)
  Gene Name
RAB7A
  Host Species
Homo sapiens
  Uniprot Entry Name
RAB7A_HUMAN
  Protein Families
Small GTPase superfamily
  EC Number
3.6.5.2
  Subcellular Location
Cytoplasmic vesicle; phagosome membrane
  External Link
NCBI Gene ID
7879
Uniprot ID
P51149
Ensembl ID
ENSG00000075785
HGNC ID
HGNC:9788
  Function in Host
Small GTPase which cycles between active GTP-bound andinactive GDP-bound states. In its active state, binds to a variety ofeffector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migrationand positioning, and endosome-lysosome transport through differentprotein-protein interaction cascades. Plays a central role, not only inendosomal traffic, but also in many other cellular and physiologicalevents, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in theaxons of neurons and lipid metabolism. Also involved in regulation ofsome specialized endosomal membrane trafficking, such as maturation ofmelanosomes, pathogen-induced phagosomes (or vacuoles) andautophagosomes. Plays a role in the maturation and acidification ofphagosomes that engulf pathogens, such as S. aureus and M. tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Playsimportant roles in microbial pathogen infection and survival, as wellas in participating in the life cycle of viruses. Microbial pathogenspossess survival strategies governed by RAB7A, sometimes by employingRAB7A function (e. g. Salmonella) and sometimes by excluding RAB7Afunction (e. g. Mycobacterium). In concert with RAC1, plays a role inregulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling ofNTRK1/TRKA. Regulates the endocytic traffickingof the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, acytosolic lipase-independent autophagic pathway. Required for the exosomal release of SDCBP, CD63 and syndecan. Required for vesicular trafficking and cell surfaceexpression of ACE2. May play a role in PRPH neuronalintermediate filament assembly. [1-7]
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  Related KEGG Pathway
Salmonella infection hsa05132            Pathway Map 
Tuberculosis hsa05152            Pathway Map 
Amoebiasis hsa05146            Pathway Map 
Mitophagy - animal hsa04137            Pathway Map 
Autophagy - animal hsa04140            Pathway Map 
Endocytosis hsa04144            Pathway Map 
Phagosome hsa04145            Pathway Map 
  3D Structure
PDB ID: 1T91 
FASTA   Download
  2D PNG Download  
PDB File Download

Function of This Protein During Virus Infection
Virus NameHCoV-229E Protein Function Pro-viral [8]
Infected TissueLiver Infection Time24 h
Infected CellHuh-7.5 Cells (Human hepatocellular carcinoma cells) Cellosaurus IDCVCL_7927 
Method DescriptionTo detect the role of host protein RAB7A in viral infection, RAB7A protein knockout Huh-7.5 Cells were infected with HCoV-229E for 24 , and the effects on infection was detected through qRT-PCR.
ResultsIt is reported that knockdown of RAB7A leads to the reduced SARS-CoV-2 RNA levels compared with control group.
Virus NameHCoV-NL63 Protein Function Pro-viral [8]
Infected TissueLiver Infection Time24 h
Infected CellHuh-7.5 Cells (Human hepatocellular carcinoma cells) Cellosaurus IDCVCL_7927 
Method DescriptionTo detect the role of host protein RAB7A in viral infection, RAB7A protein knockout Huh-7.5 Cells were infected with HCoV-NL63 for 24 , and the effects on infection was detected through qRT-PCR.
ResultsIt is reported that knockdown of RAB7A leads to the reduced SARS-CoV-2 RNA levels compared with control group.
Virus NameHCoV-OC43 Protein Function Pro-viral [8]
Infected TissueLiver Infection Time24 h
Infected CellHuh-7.5 Cells (Human hepatocellular carcinoma cells) Cellosaurus IDCVCL_7927 
Method DescriptionTo detect the role of host protein RAB7A in viral infection, RAB7A protein knockout Huh-7.5 Cells were infected with HCoV-OC43 for 24 , and the effects on infection was detected through qRT-PCR.
ResultsIt is reported that knockdown of RAB7A leads to the reduced SARS-CoV-2 RNA levels compared with control group.
Virus NameSARS-COV-2 Protein Function Pro-viral [1]
Infected TissueLung Infection Time36 h
Infected CellA549 Cells (Adenocarcinomic Human alveolar basal epithelial cells) Cellosaurus IDCVCL_H249 
Method DescriptionTo detect the role of host protein RAB7A in viral infection, RAB7A protein knockout A549 Cells were infected with SARS-COV-2 for 36 h , and the effects on infection was detected through WB.
ResultsIt is reported that Knockdown of RAB7A leads to the decreased infection compared with control group.

Host Protein - Virus RNA Network
  Network Download  

 Full List of Virus RNA Interacting with This Protien
            RNA Region: Not Specified Virus Region (hCoV-19/USA/WA1/2020 )
              RNA Region Details RNA Info Click to show the detail information of this RNA binding region [9]
              Strains Name
hCoV-19/USA/WA1/2020
              Strains Family
Alpha (B.1.1.7)
              RNA Binding Region
Not Specified Virus Region
              Virus Name
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
              Infection Cells Huh7.5 cells (Hepatocyte derived cellular carcinoma cell)  (CVCL_7927 )
              Cell Originated Tissue Liver
              Interaction Score FDR ≤ 0.05
              Method Description comprehensive identification of RNA-binding proteins by massspectrometry (ChIRP-MS)
           RNA Region: Not Specified Virus Region (hCoV-19/Not Specified Virus Strain )
              RNA Region Details RNA Info Click to show the detail information of this RNA binding region [10]
              Strains Name
hCoV-19/Not Specified Virus Strain
              RNA Binding Region
Not Specified Virus Region
              Virus Name
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
              Infection Cells Huh7 cells (liver carcinoma cell) Huh7 cells (liver carcinoma cell)  (CVCL_0336 )
              Cell Originated Tissue Liver
              Infection Time 24h
              Interaction Score log2FC = 4.1656E+14
              Method Description RNA antisense purification and quantitative mass spectrometry (RAP-MS); Tandem mass tag (TMT) labelling; liquid chromatography tandem mass spectrometry (LC-MS/MS); Westernblot

Differential Gene Expression During SARS-COV-2 Infection
GEO Accession: GSE152641
Sample Type: Blood
Samples Details: Healthy Control: 24; COVID-19: 62
Platform: GPL24676 Illumina NovaSeq 6000
  PNG Download  
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GEO Accession: GSE162835
Sample Type: Nasopharyngeal Swabs
Samples Details: COVID-19 (Mild Symptoms): 37; COVID-19 (Moderate Symptoms): 10; COVID-19 (Severe Symptoms): 3
Platform: GPL24676 Illumina NovaSeq 6000
  PNG Download  
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GEO Accession: GSE175779
Sample Type: Human Bronchial Epithelial Cells
Samples Details: Healthy Control: 4 (0, 24, 48, 72 and 96 h); COVID-19: 4 (24, 48, 72 and 96 h)
Platform: GPL18573 Illumina NextSeq 500
  PNG Download  
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Protein Phosphorylation after Virus Infection
S72 [11]
  PNG Download  
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Potential Drug(s) that Targets This Protein
Drug Name DrunkBank ID Pubchem ID TTD ID REF
Aminopterin DB08878  169371  D0D2RN  [12], [13]
Pemetrexed DB00642  135410875  D0Y4GO  [12], [14]
Trimetrexate DB01157  5583  D0Y7TS  [12]
Triparanol . 135465775  . [12]

Protein Sequence Information
MTSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVMVDDRLVTMQIWDTAGQERFQSLGVAFYRGADCCVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKASAESCSC
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References
1 Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells. Cell. 2021 Jan 7;184(1):92-105.e16.
2 Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation. Hum Mol Genet. 2010 Mar 15;19(6):1033-47.
3 Phagosomes fuse with late endosomes and/or lysosomes by extension of membrane protrusions along microtubules: role of Rab7 and RILP. Mol Cell Biol. 2003 Sep;23(18):6494-506.
4 Human VPS34 and p150 are Rab7 interacting partners. Traffic. 2003 Nov;4(11):754-71.
5 Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes. EMBO J. 2001 Feb 15;20(4):683-93.
6 Syndecan-syntenin-ALIX regulates the biogenesis of exosomes. Nat Cell Biol. 2012 Jun 3;14(7):677-85.
7 Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes. Traffic. 2011 Apr;12(4):407-20.
8 Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors. Cell Host Microbe. 2021 Feb 10;29(2):267-280.e5.
9 Discovery and functional interrogation of SARS-CoV-2 RNA-host protein interactions. Cell. 2021 Apr 29;184(9):2394-2411.e16.
10 The SARS-CoV-2 RNA-protein interactome in infected human cells. Nat Microbiol. 2021 Mar;6(3):339-353.
11 Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.
12 SARS-CoV-2-host proteome interactions for antiviral drug discovery. Mol Syst Biol. 2021 Nov;17(11):e10396.
13 Furin: A Potential Therapeutic Target for COVID-19. iScience. 2020 Oct 23;23(10):101642.
14 Prioritizing potential ACE2 inhibitors in the COVID-19 pandemic: Insights from a molecular mechanics-assisted structure-based virtual screening experiment. J Mol Graph Model. 2020 Nov;100:107697.